Biodistribution Study Of ～(131)I-GM-CSF In SCID Mice With Human Leukemia

Objective: To investigate the biodistribution of ¹³¹I-GM-CSF in SCID mice with human AML. Methods: ¹³¹I-GM-CSF was produced by chloamin-T method, was purified and verified by thin layer chromato- graphy. The xenograft model of human leukemia was established in SCID mice, was verified by the analysis of FCM, chromosome and pathology. The mice developed leukemia was divided into two groups, via tail vein, was treated with ¹³¹I-GM-CSF or the mixture of ¹³¹I and GM-CSF respectively. The biodistribution of ¹³¹I-GM-CSF in the mice with leukemia was studied. Results: 1.The labelling rate of ¹³¹I-GM-CSF was 75%, radiochemical purity was 94.1%, radioactive concentration was 2.4×10¹⁰ Bq/L and cells immune binding rate was 90.91%. 2. The inoculated HL-60 cells could grow in vivo in SCID mice, which developed leukemia in 4 weeks. 3. It is in spleen, bone marrow and tumor that ¹³¹I-GM-CSF was concentrated. In spleen and bone marrow, ¹³¹I-GM-CSF was uptaken to peak in 30 minutes after injection, and maintained at a higher level in 24 hours than the other tissue. The peak values of ¹³¹I-GM-CSF in both spleen and bone marrow were greater than the control (t=3.59,3.90, P<0.05,...