Preliminary Study On Cryopreservation Of Human Oocytes At Sifferent Stages Of Maturity

Objective:Study on cryopreservation of frozen-thawed human oocytes at different stages of maturity, by Polscope non-invasive observe meiotic spindle, analyzing the developmental potentiality of oocytes, by which to provide an experimental foundation for the safe and effective oocytes cryopreservation.Methods:The immature oocytes came from the treated patiens with in vitro fertilization-embryo transfer (IVF-ET) or intracytoplasmic sperm injection (ICSI) cycle.Oocytes were divided randomly into three experimental groups: Control Group (n=56), the oocytes were cultured in vitro maturation (IVM), observing the oocytes meiotic spindle, performing ICSI; MII frozen group (n=73), the GV and MI oocytes first were cultured IVM, then MII oocytes frozen-thawed, observing the oocytes meiotic spindle, performing ICSI; MI/GV frozen group(n=91), the oocytes were frozen-thawed first , then...