Expression Of Leptospira Interrogans OmpL1 And Preparation Of IbeA Monoclonal Antibody Of Meningitic Escherichia Coli

1. BACKGROUNDLeptospirosis, caused by Leptospira interrogans, is a wide spread zoonosis in the world. The severity of this acute febrile illness varies considerably from mild to fatal, and wide spectrum of symptoms makes clinical diagnosis very unreliable. Thus, laboratory diagnosis and prevention are therefore of the most importance for controlling the disease. In 1993, Haake and his colleagues firstly identified a full-length gene named as ompL1 with a 963bp ORFs encoding a 320 AA trans-membrane protein. By using Southern hybridization, the authors demonstrated that the ompLl gene exists in DNAs from multiple serogroups of L. interrogans. We expressed OmpL1 protein of Leptospira serogroup Ictero-haemorrhagiae serovar Lai strain using genetic engineering technique. The recombinant expressed OmpL1 of Leptospira interrogans could provide the candidate antigen for further development of diagnostic method of leptospirosis with Dipstick.Bacterial meningitis (BM) is the common severe infection of central nervons system with a high mortality and morbidity. Escherichieae coli K1 is the most common gram-negative pathogen of neonatal meningitis. Invasion of human brain vascular endothelial cell (HBMEC) is essential to the penetration of blood-brain barriers by E. coli Kl. In the present studies about E.coli meningitis, it is known that several genes may contribute to E.coli invasion of BMECs and the BBB. The ibeA...