The Study On Preparation And Photoimmunotherapy Effect Of HP-Herceptin Photoimmunoconjugates In Vitro

OBJECTIVE:To study the double directing effects of Hematoporphyrin (HP)- Herceptin photoimmunoconjugate on human breast cancer cells cultured in vitro, to determine and evaluate its killing effect on cancer cells, and promote the targeting characteristic of photodynamic therapy (PDT) and provide a more effective clinical treatment of photoimmunotherapy.METHODS:1. To subculture BT-474 cell line of human breast cancer and determine the expression efficiency of HER-2 which is located on the cell surface by flow cytometry.2. EDCI was performed to conjugate monoclonal antibody Herceptin to photosensitizer- hematoporphyrin. After dialysis and gal filtration of the conjugate, its qualitative and quantitive analyses were performed by Thin-Layer Chromatography of Carbohydrates, the UV-reflection spectrophotometry, Erythrocyte Ham's Test and ELISA.3. The killing of HP-Herceptin on BT-474 cells in vitro were determined by MTT...