Study Of C1q-Binding C7 Peptides With C1q-Inhibiting Activities

Complement system plays an important role in defense against infections and immunoregulation, however, the disorder of its activation is a major cause of tissue injury in many diseases. The anti-complement therapy has been a major field in the complement research. Different composition can be taken as target to inhibit activation of complement system. In addition to active the classical complement pathway as the target recognition protein, C1q is also involved in a number of other immunological processes including the produce of proinflamation factor via interaction with C1qR on cells. We suppose that the inhibition of C1q from interacting with immune complex or C1qR may effectively reduce the tissue injury caused by complement activation.In this research, a panel of C1q-binding phage clones was selected by screening Ph.D.-C7^? Phage Display Peptide Library with C1q, some peptides displayed on these phage clones were synthesized and their effects on complement activation were explored.Part I Selection and identification of the Clq-binding peptidesScreening Ph.D.-C7^? Phage Display Peptide Library using C1q as the target protein, a panel of C1q-targeting phage clones was recognized. The C1q-binding phage clones were identified by U937 cell-ligand-binding inhibitive assay and aggregated IgG (AIgG) competition inhibitive assay. It was found that twelve phage...