Study On Hematopoietic Promoting And Blood Lipid Regulating Function Of Sepia

The main chemical composition of sepia is melanin and protein-polysaccharide compound, which has obvious biological effects. The effects of sepia on hematophoiesis function in normal mice and in model mice of the bone marrow depression, the inhibitory effects of sepia on the transplanted tumor of Sarcinoma180 and immunoregulatoion functions, the effects of sepia on blood lipid and peroxidative levels in serum and tissue of hyperlipidemia rats were investigated.Sepia at different dose were given to normal and model mice of bone marrow depression induced by cyclophosphamide(CY) ip. or by irradiating with lethal dose of 8.0Gy gamma rays , respectively. The number of bone marrow nucleatad cells (BMNC), colony-forming units in spleen (CFU-S), colony-forming units of granulocyte and monocyte (CFU-GM) , peripheral WBC,RBC, PLT, Hb, reticulocytes and serum SOD activity in normal and the bone marrow depression model mice were measured by culturing hematopoietic progenitor cells in vitro and experimental hematology technique . The results showed that sepia could enhance the number of BMNC , CFU-S, CFU-GM and peripheral WBC in normal mice significantly , resist the decrease and promote the restoration of the indices mentionedabove in model mice of Cy and γ⁶⁰ Co irradiation obviously. But there was nosignificant effect on peripheral RBC, PLT, Hb and reticulocytes both in normal mice and in model mice. The observations suggested that sepia had significant effects on granuloppiesis. The possible mechanism was that via regulating immunological function, inducing the organism to produce GM-CSF and other cytokines, which promoted the multiplication of hempoietic cells and the differentiation to granulocyte and monocyte.