Telomere Length Measureed By Quantitative PCR

Objective: Telomeres, as the ends of chromosomes, are essential for the protection of chromosomes. In human, both in vivo and in vitro, telomere shortening appears to be a major component of cell senescence and aging. Making the measurement of telomere length, we can investigate the correlation between telomere length and aging.Methods: Peripheral blood samples were collected from healthy individuals of different age. The DNA was extracted with a reformed method of chloroform phenol. Telomere was amplified by PCR, and confirmed by ABI377. The ratio of telomere(T) PCRs to single copy gene(S) was measured using Real-time PCR.Result: Telomere fragments were amplified.Conclusion: Telomere length can be measured by Real-time PCR.