Proteomics Profile Changes In Honokiol-treated Human Ovarian Cancer Cells

OBJECTIVES: To compare the alterations in proteomes between honokiol-treated and -untreated human ovarian cancer SKOV3 cells, and to investigate the mechanism of antitumor effect of honokiol on SKOV3 cells. METHODS: SK0V3 cells were exposed to honokiol (15μg/mL) for 6 h. Then, the cells were collected and solubilized and global proteins were extracted by lysis buffer; two-dimensional electrophoresis was conducted with the IPG readystrips as carriers; the gels were stained with Coomassie blue and alterations between gels were compared by PDQuest. Eventually, 8 spots with significant differences were selected and excised and the proteins were identified by ESI-Q-TOF MS analysis.RESULTS: Exposure of SKOV3 cells to honokiol could notably increase expressions of some proteins, such as Heterogeneous nuclear ribonucleoproteins, Carbonyl reductase, Calcium binding protein, Phosphatidylethanolamine-binding protein, Keratin; while expressions of some other proteins decreased, such as Pyruvate kinase, LIM and SH3 domain, Annexin I Lipocort.