A Study Of DNA Analysis In Forensic Species Identification

Species identification plays an important role in Forensic Medicine; its main task is to identify species origins of biological samples, to determine whether the samples are from human or animal as well as to determine the species specificity when it is necessary. There are three methods applied to species identification: morphological methods, serological methods and DNA analysis. Comparing with proteins, DNA molecules are more stable, specific, and easy to analyse; therefore, DNA analysis is more helpful to identify samples with low quantity or bad quality. Species identification using DNAanalysis is the hot point in forensic medicine research. Objectives Toevaluate the species specificity of 17 new STR loci and intron 8 of the TP53 gene. To establish the methods of RFLP analysis of mtDNA cyt b gene and multiplex amplification of mtDNA — HVⅠ, HVⅡ and cyt b regions. To evaluate the values and feasibilities of these methods in case of speciesidentification of forensic biological samples. Methods 17 different kinds of ordinary animals and human being were studied in this research; DNA...