Monitoring Bcr-Abl Fusion Gene By Real Time Quantitative PCR For Chronic Myelogenous Leukemia Patients After Haemopoietic Stem Cell Transplantation

Objective To monitor bcr-abl fusion gene of chronic myelogenousleukemia (CML) dynamically, so as to evaluate those regular patterns ofcleaning leukemic clone from the CML patients by allogeneic hematopoieticstem cell transplantation (HSCT), and the correlation between dynamicchange of bcr-abl gene transcription level and disease relapse aftertransplantation.Material and Methods Bcr-abl fusion gene mRNA levels werecontinuously monitored by Real Time Quantitative PCR ( RQ-PCR) technique, and 63 samples from CML patients (including 45 from bone marrow and 18 from peripheral blood) were collected on the time points of pre-transplantation and post-transplantation. The results of RQ-PCR were compared with those of RT-PCR and cytogenetics, and combined with clinical situation, to get some message of correlation among these factors. Results There are some difference in bcr-abl/abl levels of 7 samples from CML patients at the time of pre-transplantation. The 7 patients had received different treatment during various periods respectively, resulting in different...