The Effect And Mechanism Of [alpha]-Lipoic Acid On Acute Pancreatitis Of Mice

Object To observe the effect of alpha-lipoic acid (LA) on the levels of superoxide dismutase(SOD) and malonyldialdehyde(MDA), NF-κB signal pathway and tumor necrosis factor-[alpha](TNF-[alpha]) in pancreatic tissue with acute pancreatitis (AP), to study the protective mechanism of LA on acute pancreatitis. To discover it's antiinflam-matory and antioxdative property. This results may serve as an important step toward the clinical application for acute pancreatitis patients. Methods Mice were injected with large dose L-arginine [200mg × (100g)^-1 body weight] for 2 times with an interval of 1h. All the mice were randomly divided into four groups: normal control group; AP group-acute pancreatitis group;AP+LA I group-low dose LA group [50mg ×(kg)^-1body weight]; AP+LA II group-high dose LA group[100mg × (kg)^-1body weight]. Control animals received the same dosage of physiological saline at the same time. An hour before, LA was injected intramuscularly. At 6h, 12h, 24h and 48h after injection, the animals were sacrificed. Blood samples were taken from removing eyeballs for amylase activities analysis and pancreas were removed for histological observation. The level of SOD and MDA in mice' pancreatic tissue sample of each group were assayed, the activity of NF-κB in pancreatic tissue was measuredby immunohistochemistry and intraPancreatic TNF-α mRNA expression in pancreas were studied by semi-quantitantive RT-PCR. Results The level of serum amylase was increased significantly in acute pancreatitis group than that in LA pretreated group (P<0. 05). Histologically, LA reduced the severity of pancreatic injury including inflammatory cell infiltration and necrosis at 12nd and 24th hour. The...