Studies On The Absorptive Mechanism Of Jujuboside A By Caco-2 Cell

OBJECTIVETo explore the absorption and transport mechanism of JuA in humanintestinal by uptake and transport experiment in Caco-2 cell model and to offer the theoretics and experimental gist for lucubrate of pharmacological mechanism of JuA.METHODS① Establishing the HPLC analyse method for JuA.② The establishment and evaluation of Caco-2 cell model:Because of the difference between source and culture condition, different Caco-2 cell line have different characteristic in multiplication and biochemistry. Even in the same culture condition, there are some difference between biology and bilchemistry, So the data of different laboratory only have reference worthiness, and they can not be apply mechanically. Thus we had determine some parameter such as normal microscope, transmission electron microscope, transepithelial elect rical resistance,the permeability of dextran before the establishment of Caco-2 cell model in order to insure the reliability of monolayer .③ acquainting the toxicity of JuA on Caco-2 cell by MTT method.④Seeing about the time, pH,concentration course for the uptake of JuA by Caco-2 cell, we protract the curve of culture time-uptake by Caco-2 cell. We also observe different pH, concentration and temperature course for the uptake of JuA by Caco-2 cell.⑤To study Verapamil, the inhibitor of P-glycoprotein (P-gp), Sodium azide and 2,4-Dinitrophenol ,the inhibitor of metabolism,course for the uptake of JuA by Caco-2 cell, and explore the absorption mechanism of JuA.⑥Caco-2 were seeded on Transwell and grew for about 21 days for using,...