Significance And Effect Of Curcumin On The Expression Of Composition Of The Plasminogen Activating System In Hepatic Fibrosis

ObjectiveTo investigate the effects of curcumin on the expression of urokinase-type plasminogen activator (u-PA) and Plasminogen activator inhibitor-1(PAI-1) proteins of hepatic fibrosis by carbon tetrachloride(CCl₄) in rats, thereby contributing to the role of curcumin in hepatic fibrosis and affording some theory foundation about therapy of hepatic fibrosis.MethodsOne hundred and twenty male Sprague-Dawley rats were randomly divided into six groups: normal group(N), normal control group(NC), model group(M), low dose of curcumin group (Cl), middling dose of curcumin group (Cm) and high dose of curcumin group (Ch).The rats in group of M, Cl, Cm and Ch were intraperitoneally injected with CCl₄ for 6 weeks to induce liver fibrosis, and the rats in group NC were injected with paraffin oil only. Rats in group Cl, Cm and Ch were administrated with different dose of curcumin. Random five rats in every group were sacrificed humanely at 4, 7, 21 and 42 days. Hyaluronic acid was measured by radioimmunoassay. Laminin was determined by ELISA. Liver specimens were preserved in 10% buffered paraformaldehyde and dehydrated in a graded alcohol series. Following xylene treatment, the specimens were embedded in paraffin blocks and cut...