The Relationship Between Abnormal Expression Of NPY In Hippocampus Of Intractable Epilepsy Rats And The Imparement Of Spatial Learning Memory

Objective: At present, understand that about that falling sickness studies all-round should include the cognition obstacle flaring up ,again and again and breaking out simultaneously to falling sickness. The temporal lobe falling sickness prognosis is bad, Acute falling sickness has 60-70 % to develop about after flaring up feeling embarrassed curing nature falling sickness. The relapse paroxysm and long range difficult to cure nature falling sickness use medicine to lead to the dysfunction being complicated by cognition. Already have experiment evidence indicate, the spontaneity that brain inner nerve pathological mechanism changes worsens being able to not be controlled , hippocampus nerve basic degeneration appears to escape from loss , galotinous cicatrices takes form and moss shape fibre sprouts waiting for plasticity changing being connected with after this may flare up with acute falling sickness very much. The hippocampus is falling sickness relevance brain area, Nerve peptide Y (Neuropeptide Y , NPY) is that self own is generally acknow- ledged have the anti-epileptic effect nerve peptide within the brain. Hippocampus CA1 area is the location studying .In hippocampus CA1 area cone cell tier, CA2 shapes with CA3 area also have large amount of NPY to be able to there exist in nerve basic in the tier, but the person remembers that the obstacle relation sees news report not yet with studying difficult to bring nature falling sickness concurrency under control. In this study, we made one types of learning memory deficits, animal models, and treatedthe rats with scorpion venom heat-resistant protein(SVHRP) in the therapy group.Morris water maze was used to measure the spatial learning memory ability in animals. We observed the change of NPY gene expression in hippocampus CA1 CA3 and explored the possible mechanism.Methods: Experimental rats were injected with KA (10mg/kg, 5mg/ml) subcutaneously (s.c.) to make status epilepticus (SE) model. SE latter 10 day, Use subliminal dosages KA to check falling sickness paroxysm sensibility model; SE latter 10 day , Morris water maze was to evaluate cognitive function in the animals. Changing NPY adopt immunocyte chemistry method to check the rat brain inner is expressed. After SE, Set up SVHRP at the same time curing a group and carry out observation on as said or narrated above various index.Result:1. 38 KA rats were in the stages from 4 to 5 according to the scale of Racine.2. SE latter 10 day difficult epilepsy big mouse to epilepsy stimulation sensitive obvious enhancement; SVHRP causes it to reduce obviously.3. The difficult epilepsy big mouse space study memory function obvious barrier, SVHRP causes it to reduce obviously.4. Intractable epilepsy big Rat hippocampal CA1 area CA3 area NPY immune response activeness declines obviously, SVHRP causes it to reduce obviously.Conclusion: Epilepsy correlation gene NPY area expression declines in Intractable epilepsy partner space study memory function obvious barrier big rat hippocampal CA1,CA3, SVHRP causes it to reduce obviously. After this very possible is SVHRP can reduce SE the Intractable epilepsy big rat to concern to epilepsy stimulation sensitivity.