Expression And Clinical Significance Of MIF And VEGF In Ovarian Cancer

Objective :To study the expression of MIF and VEGF in the tissue of normal ovarian tissues,benign ovarian tissues and ovarian epithelial cancer tissues and metastatic lymph node and metastatic omentum .To explore the correlation between the expression of MIF and VEGF and the ovarian cancer status, including pathological types ,clinical stage and differentiation, and prognosis. The expression and the clinical significance of macrophage migration inhibitory factor in ovarian epithelial tumor tissuesMethods: To select randomly 84 cases of ovarian specimen who were operated at the Department of Obstetrics and Gynecology at the China-Japan Union Hospital of Jilin University and chang chun Obstetrics and Gynecology hospital in 2005-2007. All the specimens of ovarian cancer were selected from the tissues without adjuvant radiotherapy and chemotherapy,including 18 cases of normal ovarian tissues,24 cases of benign ovarian tissues and 42 cases of ovarian epithelial cancer tissues and 6 cases with metastatic lymph node and 16 cases with metastatic omentum .which were divided into several groups according to pathological types, clinical stage and differentiation. metastatic lymph node or metastatic omentum. Theexpression of MIF and VEGF were detected by immunohistochemistry SP method. Results of the reactions and analysis of the clinical course of the patients were subjected to statistical analysis.Result:①The positive rates of MIF and VEGF were5.6%,11.1% in normal ovarian tissues,33.3%,41.6% in benign ovarian tumor , 66.7%,76.2% in ovarian cancer, respectively. The ovarian cancer tissues group differed significantly from normal ovarian tissues and benign ovarian tumor tissues by the expression of MIF and VEGF. And both groups significantly differed from the ovarian cancer group. A significant positive correlation was found between the expression of MIF and VEGF in the ovarian cancer group. The expression of MIF and VEGF were higher in ovarian cancer than in ovarian benign tumor. There was significant difference between two groups statistically(p<0.05).The brown granules of VEGF positive cell were mainly distributed in cytoplasm, the brown granules of MIF positive cell were mainly distributed in cytoplasm or nuclei②The positive rates of MIF and VEGF in ovarian cancer clinical stageⅠ/Ⅱwere40.0%,53.3%,that in clinical stageⅢ/Ⅳwere 81.5%,88.9%respectively. The expression of MIF and VEGF in advanced stage(III-IV) was higher than that in early stage(I-II)(P<0. 05).③The positive rates of MIF and VEGF were 81.8%,90.9% in ovarian cancer tissues with cases of metastatic lymph node or metastatic omentum 50.0%,60.0% in ovarian cancer without cases of metastatic lymph node or metastatic omentum. There was significant difference between two groups statistically(p<0.05) .④The positive rates of MIF and VEGF in ovarian cancer well differentiation and moderately differentiation were 50.0%,55.6%,that in poorly differentiation were79.1%,91.7%, respectively. The positive rates of MIF and VEGF were significantly higher in poorly differentiation than in well differentiation and moderately differentiation. There was significant difference between two groups statistically(p<0.05).⑤The positive expression of MIF and VEGF in ases of metastatic lymph node or metastatic omentum were 86.3% 86.3% ,in primary carcinoma within the ovary were81.8%,90.9%.There was significant difference between two groups statistically(P<0.05).⑥the positive expression of MIF and VEGF in serous cystadenocarcinoma were 70.8%,79.2%,that in non- serous cystadenocarcinoma were 61.1%,72.2%, there was no significant difference between two groups statistically(p>0.05).⑦There was a positive correlation between the positive rates ofVEGF and the positive rates of MIF. Conclusions:1. MIF and VEGF were low expressed in normal ovarian tissue benign tumor and were high expressed in ovarian cancer. It hints that MIF and VEGF play an significant role in carcinogenesis and growth of ovarian cancer2. The more later in clinical stage and the more poorer in cell differentiation, the more higher positive rates of MIF and VEGF were. The positive rates of MIF and VEGF were correlated with histo- differentiation and clinical stages of ovarian cancer , but were uncorrelated with the pathological type. 3. MIF and VEGF were positively correlated with the development of ovarian cancer.4. The positive rates of MIF and VEGF was higher in ovarian cancer tissues with metastatic lymph node or metastatic omentum more than without metastatic lymph node or metastatic omentum。The expression of MIF and VEGF was correlated with metabasis.5. There is no difference between the positive expression of MIF and VEGF in distant place metastasis and in primary carcinoma within the ovary.6. We can judge malignant degree of the tumor on monitoring the expression of MIF and VEGF, We still need to further study vasoformative regulation mechanism of ovarian cancer in molecular level, it will provide a new way of inhibition and interception vasoformative and future gene therapy for ovarian cancer offer theory .