The Influence Of Inflammatory Cytokines To The Expression Of Pigment Epithelium-Derived Factor

Objective To investigate the influence of inflammatory cytokines to the expression of pigment epithelium-derived factor (PEDF). Methods Three to five-passage of primary cultured human retinal pigment epithelium(RPE) cells were treated with TNF-α,IL-6 and IL-8 separately ,the concentration of the inflammatory cytokines were set at 20ng/ml,2 ng/ml,0.2 ng/ml and 0.02 ng/ml. After 6,12,24 and 48 hours , the supernatant were collected and the levels of PEDF expression were detected by western blot. Results A time- and dose-dependent decrease of PEDF expression were observed in TNF-α-,IL-6- and IL-8-treated RPE cells. Compared with the controls, the expression of PEDF decreased statistical significantly (P<0.01) after 48 hours when TNF-α,IL-6 and IL-8 was at the concentration of 20ng/ml separately. Among these three inflammatory cytokines, TNF-αwas the most strength inhibitor which downregulated the expression of PEDF by RPE cells (P<0.05).Conclusion TNF-α,IL-6,IL-8 could downregulate the expression of PEDF in cultured human RPE cells.