| The outcome of metastasis depends on the interaction of metastatic cells with different organ environments. The mechanism of organ-specific metastasis of tumor remains unknown. Present knowledge about organ-specific metastasis was derived mainly from breast cancer, melanoma and colon cancer, but little was know about liver-specific metastasis of gastric cancer. The effective regimen that can inhibit liver metastasis of gastric cancer has not been identified. In our previous study, a high liver metastasis gastric cancer cell line, XGC9811-L, was established. Taking XGC9811-L as a target, eight positive phage clones binding to target cells were identified using a peptide phage displayed library. But whether specific binding peptides could be found among them and whether these positive phage clones may play a role in liver metastasis of gastric cancer. In this study we will solve these problems.Objective Our aim of this study is to find specific binding reagents which can affect the metastasis ability of the target cells from 8 positive phage clones. The regents may provide a new approach to the therapy of liver metastasis of gastric cancer.Methods (1) Competitive binding assays were performed to test the binding efficiency of the selected candidate phages to high liver-specific metastasis of gastric cancer cells. (2) Cell ELISA and Immunofluorescent technique were used to test the cell-specificity of selected peptides and observe the binding position in XGC9811-Lcells. (3) The competitive inhibition test was carried out to determine if phage binding was mediated through the displayed peptides. (4) Cell growth assay, invasion assay, motility assay and adhension assay were performed to investigate whether the selected peptide could affect the biological behavior of... |
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