Study On Reversion Multidrug Resistance Effects And Mechanism Of Bushen Huayu Jiedu Recipe In Lung Cancer

Objective:Through observing the influence of Bushenhuayujiedu(BSHYJD) recipe to solid tumor and drug resistance lung cancer cell of DDP in lung ,we can provide objective experimental evidence for the TCM theory of enhancing kidney function & transforming stasis and resolving toxic &dissipating binding in the therapy of lung cancer and reversion multidrug resistance of lung cancer chemotherapy of Bushenhuayujiedu recipy.Methods:The model of Lewis transplanted animals were applied to study the effects of tumor inhibition and synergism to tumor-bearing animals when Chinese herbs Bushenhuayujiedu recipe combined with chemotherapeutic agents Cisplatinum(DDP). We take the lung cancer resistance cell A549/DDP for research object to observe the cytotoxicity effects of BSHYJD recipe drug-serum to the lung cancer sensitivity cell A549 and lung cancer resistance cell A549/DDP, and the influnence to cell cycle and the resistance reversion to DDP. These were determined by MTT assay. The expression of Multidrug resistance protein (MRP), Topoisomerase II (Topo II )and glutathiones transferases p(GST-p) of lung cancer multidrug resistance cell A549/DDP, cell...