Preparative Isolation And Purification Of Active Components Which Is Toxic To SARS Virus From Chinese Medicinal Herb Rheum Palmatum L

Objective:To discover anti-SARS virus active components from Chinese traditional medicinal herb Rheum palmatum L。Materials:Yew, matrine,licorice, Uvaria microcarpa, sweet tee, Auricularia polysaccharide, artesunate, Semen litchi, Brucea javanica(L.), male silk mot-hs, Luohanguo mogroside, Luohanguo tannin, bacialin, mango(Mangifera ind- ica Linn.), Rheum palmatum L, Cyrtomium fortunei are 16 species of medicinal herbs which are selected from the medicines which were commonly used in the treatment of anti-virus . Drid plant materials are ground and extracted with 75% ethanol,thus produce 16 spieces of extracts.Metheds:Thr-Ser-Ala-Val-Leu-Gln-pNA series of objects being identificated by active 3CLpro proteinase, and the cutting sequence Gln-pNA between chemical bond, after cutting the chemical bond of pNA in 405nm glazed absorption may be quantitative tests by microplate reader. Reaction in 96-well transparent plates,and containing 2.7 uM 3CLpro reorganization protein , 2%DMSO,50 mM Tris·Cl (pH 7.5), 1 mM DTT, 1 mM EDTA, and 250 uM TQ6-PN- A of the system. Response time is three hours, respectively, 0 hours, one hour, two hours, three hours measuring 405nm absorption values. 3CLpro...