| Objective Ovarian cancer is the one of the gynecology malignant tumours, because of most of the patients are advanced stage, and operation often can not completely exsect. So the chemotherapy become the principal treatment for patients with ovarian cancer. But, after chemotherapy the immune function of the organism suffered inhibit further, especially the patients of palindromia cancer and the weaknees ones, the immune function is damaged will effect the prognosis. How to relieve the contradiction, reduce the harmfulness to organism immune function maximatily, seek the optimized chemotherapy dose, has became the most important question clinically. In this study, we establish ovarian cancer animal model, and then the different dose chemotherapy (Cisplatin and Cytoxan) were carried out to the rats with ovarian cancer. We observe the immune state of parasitifer after chemotherapy to seek the parasitifer as far as possible, and increase the immune state of parasitifer, and provid reference for clinical application of the low dose chemotherapy.Method Poorly differentiated epithelial cell line NuTu-19 of Fischer 344 rat ovarian cancer was cultured in vitro. Then, we established ovarian cancer metastasis models by abdominal cavity injection 55 in inbred line Fischer 344 rats for 5×10~6 cancer cells every. Different dose Cisplatin and Cytoxan were carried out to heal the rats of randomization. After the therapy, we taked the peripheral blood, MTT method was used to detect the activity of the NK cell, and then putted the rats to death , took the metastatic tumor tissues. Histochemistry essay was used to detect the distribution and quantity of tumor infiltrating lymphocyte .Result (1) All the animals were displayed inequality hemorrhagic ascites and metastatic tumor focus of infection in abdominal cavity.(2)After chemotherapy, the third day and the fifth day was the best action time of DDP and CTX, so it was considered the experiment observe time. (3) The activity of the NK cell of the simplex cancer rats was significantly lower than the normal rats (P<0.05). (4) The number of CD4~+ CD8~+ in abdominal cavity metastatic tumor is significantly higher than the nomal ones (P<0.05), and the number of CD4~+, CD8~+ and the ratio (CD4~+/CD8~+) in the cancer nest were significantly lower than those in the stroma (P<0.05). (5) The activity of the NK cell and the ratio (CD4~+/CD8~+) in the cancer nest of the 0.3mg/100g Cisplatin group was significantly higher than before chemotherapy and others groups (P<0.05); 5mg/100g Cytoxan group was significantly higher than before chemotherapy and others groups too(P<0.05); and the activity of the NK cell and the ratio (CD4~+/CD8~+) in the cancer nest of Cisplatin was higher than Cytoxan significantly (P<0.05).Conclusion (1) The cellular immune function of the rats with ovarian cancer were significantly lower than the normal rats, and the large dose of Cisplatin and Cytoxan can made the immunological activity descend further.(2) The low dose of Cisplatin(0.3mg/100g) and Cytoxan(5mg/100g) can stimulate the immunological activity of the tumor host, the immune enhancement effect appearced the third day and the fifth day after the abdominal cavity injected respectively and keep on about 2 days. (3) The low dose of Cisplatin may stronger than Cytoxan in immunological activation capability , so the low dose of Cisplatin may beneficial to the advanced stage and weakly patients. |
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