| Objective: Dry eye is the most common ocular surface disease now, the cause of a disease is various and the pathological process is complicated. Along with the development of social information, the incidence rate of dry eye disease has ascending tendency year by year. Now the dry eye disease has already become an disregardful public health problem, and has been paid more and more close attention to it. Due to the various of etiopathogenisis of the dry eye disease, and the clinical represent without specificity, these bring the maximum difficulty to the clinical diagnosis and treatment. Especially in treatment aspect often use tear fluid instead and retain tear fluid persistence in eye as far as possible, but we has not yet treated potential course of the dry eye disease. Now some scholars think that the inflammation of ocular surface is an very significant factor which influences dry eye pathogenetic condition .The most scholars prone to make this type of inflammatory reaction act as various kinds dry eye common pathogenesy. This experiment developed a mouse model of dry eye, and then observed the tear production and corneal fluorescein stainning, meanwhile, immunohistochemical analysis were performed to detect inflammatory cytokine expression of lacrimal gland. This presents experimental evidences for antiinflammatory clinical therapy of dry eye .Method: twenty healthy female SD rats (8w age, 200-250g weight), after anesthetization, under operating microscope ,all right eyes received injection 0.05ml(20mu) of BTX-A and left eyes received saline 0.05ml into the lacrimal gland inspectively. Tear production and corneal fluorescein staining were evaluated in all rats before injection and at 3d,1w, 2w, 4w, 6w time points after injection. Corneal fluorescein staining was photographed with digital camera fitted with a macro lens. After injection the rats were removed all lacrimal gland and put in 4% Paraformaldehyde, fixed for 24 hours, HE staining and immunohistochemical technique were applied.Results: BTX-A injected rats were observed light hyperaemia in conjunctiva from 2w, bulbar conjunctiva defects luster, some appears reductus. After 1w, corneal punctiform epithelium stripping and corneal fluorescein staining. Meanwhile the tear production were measured with phenol red-impregnated cotton threads.The reduction in tear production persisted for 4 weeks in all treatment groups after BTX-A injection, then tear production had already returned to normal levels. However, the control group showed no corneal staining and no obvious change in secretion of tears. Compare with the before injection, the tear production decreased in 4 weeks, until 4w time points, no statistically significant differences. There is no corneal fluorescein staining before injected BTX-A, while after injected showed amounts staining from 1w, the staining degree became deeper and deeper along with the time, there is no staining in all control eyes all the time. All BTX-A injected lacrimal gland displayed the notive IL-6,IL-1β,TNF-α, after 4 weeks, control group did not appear IL-6,IL-1β,TNF-αin all the time.Conclusion: In this study, we injected BTX-A into lacrimal gland and reduced secretion of tears,established the dry eye clinical model. Meanwhile we researched the BTX-A induced animal model at several time points and fortell the inflammatory cytokine expression in lacrimal gland of dry eye. This point that inflammatory reaction in pathogenetic process of dye eyes. This provided pathogenetic and clinical antiinflammation of therapy of dry eye more ideal experimental foundation. . |
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