| Background and Aim: The genesis of gastric cancer is accumulation ofmulti-factor, multi-stage and multi-gene abnormality, among them, inactivation of suppressor gene which induced by promoter hypermethlation is a important mechanism of the genesis and development of gastric cancer. Runx3 gene is a new suppressor gene of gastric cancer specificity. In our study, we analyze the relations among the aberrant methylation of the promoter of Runx3, its protein expression, and the clinicopathological features of gastric cancer. Further to discuss whether Runx3 can be a new target of diagnosis and treatment of gastric carcinoma.Methods: Methylation specific PCR was used to measure the promoter methylationstatus of Runx3 gene of the tumors and the adjacent normal mucosal tissues of 40 patients with gastric cancer. Protein expression of Runx3 was measured by immunohistochemistry. The relationships between Runx3 expression and clinicopathological parameters were analyzed.Result: The frequency of promoter methylaion of Runx3 gene in gastric cancer(55.0 %,22/40)was higher strongly than the adjacent normal tissues(12.5%,5/40)(p<0.01);the rate of protein expression of Runx3 in gastric cancer (37.5%,15/40) was lower significantly than the adjacent normal tissues (100%,40/40) , and there was marked association between hypermethylaion and negative protein expression (p<0.05) . There was significant correlation between the frequency of promoter methylaion of Runx3 gene and histological type, Ngrade,or tumor stage (P＜0. 05～0. 01) .Conclusion: The promoter hypermethylation is a main mechanism of reduced andloss expression of Runx3 gene, and it may provide an evidence for molecular diagnosis and stage evaluation of gastric cancer.
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