The Experiment Research On The Relativity Between Coronary Atherosclerosis And Elasticity

Background:Coronary heart disease is one of the important factors of human health and socio-economic impactions. About 99% of coronary heart diseases are due to atherosclerosis. Atherosclerosis are currently considered to be inflammatory diseases progress, its characteristic pathological changes of endometrial plaque formation and arterial wall hardening and the pathological mechanisms are not clear. Therefore, the incidence and development of early detection mechanism, prevention and control measures in the field of medical research has been a major topic. Early atherosclerosis in the arteries flexible change reflects the extents of coronary atherosclerosis. For further research and early identification and early prevention of atherosclerosis is very significant. COX-2 is involved in inflammatory response isomerase. Various studies show that COX-2 is in atherosclerotic lesions in the early stages of the development. Recordings of NF-κB signal transduction in cells is an important transcription factor involved in the inflammatory process of a variety of signal transduction pathways as arterial wall was activated. The extracellular matrix of collagen is not only the atherosclerotic plaque stent, but also have an important role to play in the process of atherosclerotic lesions. Arterial Elasticity of the artery wall is buffering capacity, reflecting the state of arterial diastolic function,which depends on the artery wall cavity size and hardness. Its normal function depends on the composition of arterial structure and function of the normal volume. Pulse wave velocity in the arterial pulse wave is the established system and the point spread. According Moens-Korteweg equation. Pwv depends on the size of the biomechanical properties of arteries (arterial elasticity) , vascular geometric features (lumen diameter and wall thickness) and blood viscosity. Because vascular changes in blood viscosity and the geometric characteristics is of a relatively litter Variation,PWV has become the classic indicators of arterial structure and function. Statins are drugs effective lipid-lowering drugs, has also confirmed that he has a direct anti-inflammatory effects of statins drugs. AS formed in the process of improving its function of arterial elasticity to be confirmed. Amlodipine is the third generation calcium channel blocker, is currently thought to have a clear anti - atherosclerosis, But the impact of artery atherosclerosis flexibility is not yet clear. The experiment is succeeded in establishing a high-cholesterol diet induced rabbit model of AS, arterial specimens were observed. Compared to atorvastatin, we aim to explore the role of amlodipine improving arterial elasticity. Futher , we try to analysis the correlation between pulse wave velocity(PWV) and coronary atherosclerosis.Materials and Methods:Twenty-four male New Zealand rabbits (3 months old)were fed with normal diet (n = 6) and high-cholesterol diet ( n = 18) respectively for 4 weeks , and then the high-cholesterol diet rabbits were assigned to receive either atorvastatin 2. 5 mg/ (kg·d) (n = 6)or Amlodipine ( n =6 ) . Experimental testing process for the following : (1) at the start of the experiment after experiment, and one day before the 12th week, fasting venous blood from 6-8 ml rabbit ears measuring total cholesterol (TC). triglyceride (TG), low-density lipoprotein cholesterol (LDL-C) (2) prior to the test, one-day and the end of the experiment at 30 kg intravenous injection of sodium pentobarbital anesthesia ear, neck operation in accordance with the conventional surgical incision Outside pressure mercury blood pressure measurement devices. Fixed at the stage of animal rabbit anatomy, weight, blood pressure, French companies in the development and production of rabbits input pulse wave velocity detector -home-Sport Pollock (Complior SP) simultaneously rabbit carotid-femoral PWV determination, and (3) coronary artery and aortic pathology. (4) Application Masson arterial collagen content was measured. (5) immunohistochemical analysis of COX-2 protein and NF-κB protein in coronary artery and aortic are Recorded.Results:Compared with the control group, the degree of the atherosclerosis stenosis of coronary artery branches and pwv were significantly increased in model group(P<0.05).COX-2 protein expression , NF-κB protein expression and collagen accumulation in plaques from atherosclerosis rabbit models were significantly increased compared with those from normal rabbits , and significantly reduced by the treatment of atorvastatin and amlodipine (both P < 0. 05) . Pwv is related to The degrees of the atherosclerosis stenosis and the levels of COX-2 protein expression , NF-κB protein expression in atherosclerostic plaques( both P < 0. 05).Conclusion:1 Pulse wave velocity was significantly correlated with coronary atherosclerosis in the early stage of atherosclerosis. 2 Hypercholesterole-induced inflammatory response in the early formation of atherosclerosis; lesions of COX-2 protein expression was significantly increased in early atherosclerosis of hypercholesterolemic rabbits; 3. Recordings of COX-2 and NF-B in the early formation of atherosclerotic lesions promote, The expression reflects the extent of atherosclerotic lesions. 4. atorvastatin and amlodipine early atherosclerotic lesions inhibit COX-2 protein expression; 5. Atorvastatin and amlodipine may play a role of improving arterial elasticity through the inhibition of COX-2 anti-inflammatory effects.