The Expression Of Ang-2 And It's Relationship With Microvessel Density In Epithelia Ovarian Carcinoma

Ovarian carcinoma is one of three malignancies in female reproductive tract, in which 60%~90% is composed of epithelia ovarian carcinoma。the 5-year survival rate of epithelia ovarian carcinoma is about 30% and the reasons of its'origin and development have been not clear. Ang-2 is discovered as an important adjustor to angiogenesis recently. Overseas research indicates that the expression of Ang-2 is a factor of angiogenesis,origination and enhance of tumor, related tightly to microvessel count ,clinic stages and prognosis. Tumor microvessel formation might be early events of origination and development in ovarian carcinoma. The fuction of Ang-2 in epithelia ovarian carcinoma has been not reported in domestic. Objective This research investigates the expression of Ang-2 in normal postmenopause ovary and epithelia ovarin carcinoma, and counts the microvessel density of tissue signed by CD34, and discuss the relations of Ang-2 and clinicpathological features of epithelia ovarin carcinoma, hence discover the fuctions of Ang-2 in angiogenesis and development of epithelia ovarian carcinoma. Methods 46 paraffin specimens was random selected from operated patients with epithelia ovarian carcinoma in our hospital from Jun,2005 to Dec,2006, and 15 paraffin specimens of normal postmenopause ovary was random selected from operated patients with other diseases. All selected patients didn't recepted hemotherapy,ridiotherapy or immunity therapy. The expression of Ang-2 and CD34 was detected by immunohistochemistry SABC, and counts the MVD of tissues signed byCD34. All datas is dealed with SPSS statistic software. Results (1) The positive expression rate of Ang-2 in epithelia ovarian carcinoma is 39.1% and 6.7% in normal postmenopause ovary, there is significant differences (x2=4.15,P<0.05); The MVD in epithelia ovarian carcinoma group: 28.33±8.6, the MVD in normal postmenopause ovary group: 10.64±1.58, there is significant differences (t=7.88,P<0.01)。(2) The positive expression rate of Ang-2 in theⅠstage andⅡstage of epithelia ovarian carcinoma is significantly lower thanⅢstage andⅣstage(x2=6.175,P<0.05); The MVD in theⅠstage andⅡstage of epithelia ovarian carcinoma is significantly lower thanⅢstage andⅣstage(t=4.67,P<0.01). The expression of Ang-2 and the MVD in epithelia ovarian carcinoma is not correlated with types and degrees of histology. (3) In epithelia ovarian carcinoma, MVD in positive Ang-2 groups is :36.11±6.42, MVD in negative Ang-2 groups is :23.33±5.58, there is significant differences(t=7.16,P<0.01). Their was a remarkable positive correlation in the expression of Ang-2 and MVD(r=0.733). Conclusion The expression of Ang-2 might be related to the development and metastasis of epithelia ovarian carcinoma, and may promote the development and metastasis of epithelia ovarian carcinoma by promote angiogenesis in tumor.