The Correlation Study Of Liver Type Fatty Acid-binding Protein And Chronic Kidney Disease

Objective: To investigate the relationship between L-FABP and CKD, We assessed the clinical parameters of urinary L-FABP, serum Scratinine, total cholesterol, triglyceride, urinary and serumβ2-MG, urinary protein and glomerular filtration rate,Methods: There are two groups in experiment: without liver injury CKD group (23cases) and healthy group (23 cases). To obtain serum and urinary samples of studied objects, measure the level of urinary L-FABP, serum Scratinine, total cholesterol, triglyceride, urinary and serumβ2-MG, urinary protein and glomerular filtration rate using serum and urine from CKD and healthy group. urinary L-FABP was measured using human L-FABP ELISA kit (ADL, co, Ltd, USA). centrifuge the urine and take the supernatant. Prepare the ringing buffer by diluting the wash buffer 20×, diluting the sample diluent5×, dilute samples1: 100 distribing 5μl of urine into 500μl of diluent.Place 100μl of diluted sample/reagent blank/standard/controls to the wells of the strips.Incubate for 30 min at 37℃, wash 5 times. Then add 100μl of enzyme conjugate to each well, incubate for 30 min at 37℃, wash 5 times. Again add 50μl color reagent A and color reagent B to each well, incubate for 15 min at 37℃. Eventually add 50μl stop solution. read absorbance at 450nm within 15 min. Construct a standard curve by plotting the mean absorbance obtained for each reference standard against its concentration on linear graph paper, determine the corresponding concentration from the standard curve.All measurement data are expressed as the mean±standard deviation (SD). These statistical analyses were done by Stat View software (SAS Institute Inc, NC, USA). The correlation between two groups analyzed by the Mann-Whitney T-test or the Wilcoxon rank-sum test. P values less than 0.05 were considered statistically significant. The correlation was analyzed by Pearson'rank coefficient of correlation.Results: Urine excretion of L-FABP in CKD groups was less than in healthy group (P<0.01). There was no certain correlation between urinary L-FABP and Scr,GFR,upro,serumβ2-MG,urineβ2-MG,Cho,TG by use of Pearson'rank coefficient of correlation.Discussion: CKD has become a worldwide public healthy problem. The incidence rate of renal failure gradually inScrase with poor prognosis and high spend. It is very important of early discovering renal injury, monitoring progression and prognosis judgement. Urinary L-FABP might be a new useful clinical biomarker for diagnosing CKD and monitoring its progression. L-FABP is expressed in both kidney and liver. It is possible that serum L-FABP derived from the liver influences urinary L-FABP, we therefore excluded patients with liver damage. There are radio-immunity, ELISA and immunoturbidimetry to determin urinary L-FABP,some molecular biology technique such as nucleic acid hybridization, PCR, RT-PCR et al show up a moment using prospect at gene aspect. We determine urinary L-FABP by frequently used ELISA method at present.Many study confirm that tubulointerstitial damage plays a important role in the progression of CKD. A lot of recent clinical and experimental studies indicate that the extent of tubulointerstitial damage is the capital marker for monitoring the severity degree of renal function decreased and judging prognisis in various kinds of primarily, secondary renal glomerular disease and negative renal glomerular disease (such as chronic pyelonephritis and chronic obstructive nephropathy). The fuse of renal tubule inflammatory reaction or cell damage may be the compound bound to albumin like as free fatty acid (FFA), the albumin bound to FFA possess activity of inducing macrophage, but degrease albumin doesn't. FFAs are bound to cytoplasmic FABP in the proximal tubule and transported to mitochondria orperoxisomes, where they are metabolized byβ-oxidation. In massive proteinuria, FFAs are overload in the proximal tubule and induce inflammatory factors such as macrophage chemotactic factors, which cause tubuloinstitial damage. In this study, urine exscretion of L-FABP in CKD groups was less than in healthy group (P<0.01). But due to the limitation of experiment cycle and sample number, the correlation between urinary L-FABP and other index such as GFR, Scr, upro, serumβ2-MG and urinaryβ2-MG does not definite, we should carry out more study in the future.In a word, as a new clinical biomarker, urinary FABP has consanguineous relations with tubulointerstitial damage, it also has extensive application prospect in diagnosis, differential diagnosis, monitoring proceeding, prognosis judgement and therapy of chronic kidney disease.Conclusion: urinary L-FABP decreases significantly in chronic kidney disease. As a clinical biomarker, urinary L-FABP can help to diagnose chronic kidney disease. The correlation between urinary L-FABP and other index such as GFR, Scr, upro, serumβ2-MG and urinaryβ2-MG do not definite.