| BACKGROUND and OBJECTIVEThe morbidity and mortality of Coronary Heart Disease(CAD) gradually increase nowadays. Ventricular remodeling is a major cause of progressive heart failure and death after myocardial infarction. As adaptive reaction to myocardial ischemia and one of remodeling integral processes, body can accelerate the creation of coronary collateral circulation by proliferating capillary vessel. But in most conditions, this compensatory process is insufficient. Accelerating the formation and open of coronary collateral circulation can ameliorate relieve ischemia of myocardium and relieve ventricular remodeling. Bone marrow stromal cell is multipotential stem cell which has the potential ability to induce angiogenesis in ischemic tissue. So we transplanting bone marrow stromal cells into swine infarct area to observe its effect to angiogenesis and heart function after swine myocardial infarction, and investigate the improving effect of intramyocardial transplantation of autologous bone marrow mononuclear cells on the cardiac function of swine with chronic ischemic myocardium.METHODS1).We created a novel model of myocardial infarction in swine by embolization of the left coronary artery using a gelatin sponge through cardiac catheter.2).At the same time, 10-15 milliliter bone marrow was aspirated from the postern- superior iliac spine of swine. The mesenchymal stem cells (MSCs) were cultured according to the methods of Ficoll's.3).After myocardial infaraction, dodeca swines were equally divided into experimental group (B group)and control group (A group) randomly. The same volume of the MSCs or culture medium were transplanted by intramyocardial injection through cardiac surgery operation.4).Four weeks after implantation, The heart function of those swines were evaluated with echocardiogaphic and SPECT cardiac blood pool examination at 3 days before the tranlplantation, 4 weeks after the transplantation. After the last examination, the animals were killed and the hearts were harvested. In the end, swines heart were excised to make pathological sections, basic changes were examined by H.E stain. Vessel number was counted in experimental group and control group by Immunohisto-chemistry study with CD31 monoclonal antibody.RESULTS1. Left ventricular function, including ejection fraction (EF), left ventricular end-systolic dimension (LVESD), left ventricular end-diastolic dimension (LVEDD), ets. was significantly improved in B group when compared with A group. (P<0.05)2. The quota of left ventricular ejection fraction(EF), 1/3 left ventricular ejection traction(1/3EF), 1/3 left ventricular filling fraction(1/3FF) in the experimental group were better than those in the control group. (P<0.05) 3. Immunohisto-chemistry study showed that vessels stained with CD31 MAB was much more than control group. (P<0.05)CONCLUSIONS1). The model of myocardial infarction is reliable, reproducible, andsimilar to the human condition, amenable to investigate otherinvestigation.2).The therapy of implantation of autologous mesenchymal stem cells byintramyocardial injection through cardiac surgery operation waseffective, feasible and safe in ischemic heart disease.3).Tranlplantation of MSCs can improve the cardiac function ofischemia myocardium of swine.4).Implantation of MSCs seems to improve the cell viability and bloodperfusion in the infracted area, indicating that MSCs induceangiogenesis. |
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