| ObjectiveTo investigate the effect of glucose and glucocorticoid on theexpression of 11β-HSD1,11β-HSD2 gene transcriptions in PIEC invitro,and the relationship between 11β-HSD1 and transforming growthfactor-β1 and Endothelin-1.Meanwhile,to observe the effect of 11β-HSD1,11β-HSD2 antisense oligonucleotides on the expression of 11β-HSD1,11β-HSD2 by PIEC under hyperglycemia.MethodsPIEC were cultured in medium in vitro under different concentrationof glucose(5.6mmol/L,10mmol/L,20mmol/L,30mmol/L)and in differentconcentration of glucocorticoid and antisense oligonucleotides underhyperglycemia. RT-PCR technique was applied to determine theexpression of 11β-HSD1,11β-HSD2,TGF-β1 and ET-1mRNA, western-blot technique was performed to measurer the expression of 11β-HSD1,11β-HSD2.Results1. Treatment of different concentration of glucose for 48h, the expressionof 11β-HSD1, TGF-β1 mRNA under high glucose condition weresignificantly increased and represent a certain correlation; high glucosecan increase the expression of 11β-HSD1 protein, both mRNA and protein of 11β-HSD1 and 11β-HSD2 were blocked by 11β-HSD1,11β-HSD2 antisense oligonucleotides; meanwhile, the expression ofTGF-β1 mRNA was decreased.2. Under hyperglycemia, glucocorticoid can upregulate the expression of11β-HSD1 mRNA and protein.the expression of mRNA and protein of11β-HSD2 were not have a significant change.ConclusionsHigh glucose and glucocorticoid can increase the expression of11β-HSD1 mRNA and protein, there is a certain linear relationshipbetween the expression of 11β-HSD1 and TGF-β1 mRNA.11β-HSD1may have a relationship with diabetes mellitus. |
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