The Polymorphism And Significance Of GRIN2A Gene's Two SNP

InductionGlutamate (Glu) is a very important excitability amino acid in human's metabolism,and it's the most abundant excitability nerve matter in the nerve centre of mammalian. Glu participates in many important functuinal adjust of nerve system. When the glutamate receptor is activated,it may impulse the effect-actor to change the potential of cell membrance. Glu makes role effect in human study memory and nerve transmit.Glutamate receptor is divided into two kinds: ionotropic glutamate receptor (iGluR) and metabotropic glutamate receptor (mGluR). According to the difference of activator, iGluR is divided into NMDAR (N-methyl-D-aspartate receptor) AMPAR KAR (kainite receptor).NMDAR includes two parts: NR1 and NR2. NR2 can be divided into 4 kinds NR2A NR2B NR2C NR2D.The NR2A is also called GRIN2A. GRIN2A gene is located in16p13.2 and is 6293bp long. There are so many variance which can be inherited in GRIN2A gene and the number of SNP is large.Our research is mainly about the polymorphism and significance of GRIN2A gene's two SNP: rs17750303 (A→C) and rs837690 (A→G) .These two SNP are located in the 3 intron of GRIN2A gene. We investigate the gengtic polymorphism of the 2 SNP in Northern Chinese Han. Further more approach the association between rs17750303-rs837690 and schizophrenia.Material and MethodsOrdering the primer by analyzing the aim-sequence, we use PCR method to amplify the GRIN2A gene rs17750303-rs837690 locus,and use BstNI restriction endonucleases digest them, by PAGE followed silver stain. We genotype the genes of 139 Chinese han.ResultIn 139 Chinese han individuals, we find two alleles and three genotypes in each SNP locus. Rsl7750303: homozygote AA was 31 individuals, its frequency was 0.223; homozygote CA was 60 individuals , its frequency was 0.432;homozygote CC was 48 individuals, its frequency was 0.345.Allele C frequency was 0.561 , allele A frequency was 0.439.Rs837690: homozygote AA was 38 individuals, its frequency was 0.273; homozygote GA was 50 individuals, its frequency was 0.360;homozygote GG was 51 individuals, its frequency was 0.367.Allele G frequency was 0.547 , allele A frequency was 0.453.DiscussionThis study investigated the GRIN2A gene 2 SNP(rs17750303 rs837690) polymorphism in the Chinese Han, by statistics analysis, and the data obey the Hardy-Weinberg regulation. According to the rs17750303 genotype and allele frequency in Chinese Han data, we conclude that the DP value is 0.645, the H value is 0.493, the EPP value is 0.187 . According to the rs837690 genotype and allele frequency in Chinese Han data, we conclude that the DP value is 0.661, the H value is 0.496, the EPP value is 0.186 . This loci is highly discrimination power genetic system.To compare the Chinese Han individual with the Northern American individualof GRIN2A gene rs17750303, the allele frequency distribution has a obviouslystatistic difference, P<0.05.Conclution1. Our study report the genotype frequency distribution of GRIN2A gene rs17750303 and rs837690 locus in Chinese Han individuals, and provide the necessarily data in forensic medicine. The Chinese han individuals have highly discrimination power and EPP.2. The GRIN2A gene rs17750303 locus frequency has obviously difference between the Chinese Han individual and the Northern American individual (P<0.05).