Investigation On Extraction, Components Analysis And Pharmacology Of Lipids From Black-bone Silky Fowl

Black-bone silky fowl is a traditional Chinese medicine. The extraction, components analysis and pharmacology function of lipids from Black-bone silky fowl were investigated in this paper, which will provide scientific functions for their further developments as active ingredients in functional food, or new medicine. The main studies and results in the paper are mentioned as follows:New extraction technologies of mixed solvent extraction designed by Box-Behnken Designs were applied in extracting lipids of Black-bone silky fowl, which examined by the yield of lipids. Based on these, response surface methodology was carried out and the following optimum parameters technology parameters were obtained: 73℃of extraction temperature for 60 min, 1:6.6 of ration of mixed solvent. The yield of lipids was 9.34% under optimum parameters, which accounted for 98.52% of CM extraction method.External appearance of Lipids of Black-bone silky fowl extracted by mixed solvent method is flavescens semiopaque semisolid. The physico-chemical property of lipids were acid value 4.367, saponification value 175.9, peroxide value 0.11, iodine value 124.3. And the content ofα-tecopherol was 25.41+0.47μg/g lipids in black bone silky fowl, it significantly higher than that in Chongren chicken which were under the same breeding conditions.An o-phthalaldehyde (OPA) colorimetry method was developed for the determination of cholesterin in lipids of Black-bone silky fowl. The results showed that the method was simple and dependable, and the color of the treated samples was stable within 4 h. The cholesterin content in lipid of Black-bone silky fowl was 1.29mg/g, which amounted to 12.29 mg/100 g in fresh chicken.The fatty acids composition of lipids from Black-bone silky fowl was determined by GC/MS. The results showed that the black-bone silky fowl lipids was characterized by high contents of unsaturated fatty acids, and the ratio of monounsaturated fatty acid/saturated fatty acid/polyunsaturated fatty acid was nearly 1:1:1.Lipids from Black-bone silky fowl can obviously increase the level of WBC, RBC,HB of both blood losing and haemolyticus model mice, raise the body temperature, physical strength and thymus index of haemolyticus model. It suggested that lipids from Black-bone silky fowl had good effects on blood-supplement.Lipids from Black-bone silky fowl could significantly increase the water, Hyp and collagen contents in derma of old KM mice compared with lipids from Chongren chicken.