Observation On DNA Damage And Adverse Effects Of Radiotherapy To Esophagus Tumor By Application Of SCGE, MNT And FCM

Objective:By using the methods of SCGE(Single Cell Gel Electrophoresis),MNT (Micronucleus Test),and FCM(Flow Cytometry)respectively,DNA damage,adverse effects and variation situation of T lymphocyte subgroups in the peripheral lymphocyte of esophaus tumor sufferers after radiotherapy are measured;and the causality between radiotherapy dose and DNA damage degree to normal histiocyte are studied.The objective is to measure the DNA damage degree to normal histiocyte,the influence to immune ability of body of esophagus tumor sufferers after the radiotherapy.By means of this effect evaluation,more reliable quantitative change on clinical biology is studied.Methods:By using the methods of SCGE and MNT,DNA damage situation in the peripheral lymphocyte of 36 cases in different radiotherapy stages are measured. Meanwhile,by using the method of FCM,variation situation of the T lymphocyte groups of them in different stages are measured.Results:1.As to the SCGE measurement,the rate of length migration of the peripheral lymphocyte in healthy groups was 18%,averaged tail length was 0.88±0.05;the rate of length migration in patient groups before radiotherapy was 22%,averaged tail length was16.42±1.53;when the radiation dose was 20Gy,the rate of length migration was 44.6 %,averaged tail length was33.74±4.26;when radiation dose amounted to 30 Gy,the rate of length migration was 50.5%,averaged tail length was35.12±3.45;when radiation dose reached to 40 Gy,the rate of length migration was73.9%,averaged tail length was 41.83±2.36.2.As to the MNT measurement,the micronucleus cell rate of this sample was 0.66‰before the radiotherapy,and was 1.81‰before the radiotherapy.3.As to the immune function measurement,when they are diagnosed the esophagus tumor sufferers, their immune function of T lymphocyte subgroups were a little abnormal,that was,the proportion of the CD3~+,CD4~+,andCD4~+/CD8~+ was decreased while the CD8~+ was normal;but during the radiotherapy the proportion of the CD3~+,CD4~+and CD4~+/CD8~+ was decreased more.Conclusion:1.The different radiation dose can lead to the DNA damage of in the peripheral lymphocyte of the esophagus tumor patients;and the damaging degree varies according to difference of dose.2.Radiotherapy can reduce their immune function of the esophagus tumor sufferers in different degree.3.Different radiation dose may result in adverse effects in different degree.4.This study showed that the measurement to DNA damage of in the peripheral lymphocyte of the esophagus tumor sufferers,as well as that to T lymphocyte subgroups can provide some references on effect evaluation of radiotherapy to esophagus tumor.Therefore,more deeper discussion is necessary.