| Objective: Primary liver cancer is one of the most common malignant tumors in our country. The surgical treatment is still the most important way to manifest improve the survival rate of the patients at present, but the survival rate still remain lower. It is correlated with the bionomics of easy invasion and metastasis in hepatocellular carcinoma. It is reported that lymphatic metastasis and hematodes metastasis are frequently associated with metastasis and recurrence of hepatocellular carcinoma.Vascular endothelial growth factor-D (VEGF-D) is a new members of the vascular endothelial growth factor VEGF family of cytokines. It is reported that VEGF-D has lymphangiogenic actions in cancers and can promote lymph node metastasis metastasis. The VEGF-D/ flt-4 signal pathway is correlated with lymphatic metabasis in supraglottic carcinoma, uterine cervical cancer, ovaries cancer, large intestine carcinoma, breast cancer, but few reported that VEGF-D is closely involved in the invasion and metastasis of hepatocellular carcinoma. By testing the expressions of VEGF-D and counting flt-4 positive number in primary hepatocellular carcinoma tissues, paratumor tissues and normal liver tissues, to determine the relationship between theformer factors and lymphatic regen and the feature of metastasis in hepatocellular carcinoma tissues.Methods: Pancreatic cancer tissue samples were obtained from fourty-eight patients and twenty-eight tumor-surrounding tissue samples undergoing surgical resection. Twelve normal liver tissue samples were taken from previously patients with benign tumor. S-P method of Immunohisto -chemistry was used to detect the expression of VEGF-D and flt-4. Statistical analysis was used to determine the relationship between their expression and clinical pathological parameters.Results1 VEGF-D immunoreactivity was present in the cytoplasm of cells in hepatocellular carcinoma, VEGF-D immunoreactivity was present in thirty-six (75.0%), only in fifteen of twenty-eight tumor surrounding tissue (50%). Five of twelve (41.7%) VEGF-D positive stained in normal liver tissue samples was observed and VEGF-D positive stained rate was higher in tumor tissue than that in tumor-surrounding tissue and in normal liver tissue (P<0.05).2 Numerical value of flt-4 positive number was 17.82±5.03 in tumor tissue samples, 12.41±4.16 in tumor-surrounding tissue samples and 4.06±0.91 in normal liver tissue samples . Numerical value of flt-4 positive number was significantly higher in tumor tissue than that in tumor-surrounding tissue and normal liver tissue (P<0.05) Numerical value of flt-4 positive number was 22.60±4.15 in lymphatic metastasis,17.22±3.76 in not lymphatic metastasis. Numerical value of flt-4 positive number was significantly higher in lymphatic metastasis than that in no lymphatic metastasis in carcinoma.3 Numerical value of flt-4 positive number was 18.73±4.02 in VEGF-D-positive number stain tumor tissue samples and it was 13.57±4.72 in VEGF-D-negative stain tumor tissue samples. Numerical value of flt-4 positive was higher in VEGF-D-positive stained tumor tissue than that in VEGF-D -negative stain tumor tissue (P<0.05). VEGF-positive stain was uncorrelated with age, sex, the tumor size, AFP level and the level of tumor differentiation (P>0.05), but it correlated with intrahepatic or lymphatic metastasis, portal vein or biliary duct tumor thrombosis (P<0.05).Conclusion1 VEGF-D are commonly overexpressed in pancreatic cancer than in para-carcinoma tissues and normal tissue. VEGF-D immunoreactivity was present in some the cytoplasm of cells and contribution to age, sex, the tumor size, hepatic function, AFP level and the level of tumor differentiation, but it correlated with intrahepatic or lymphatic metastasis, portal vein or biliary duct tumor thrombosis, which indicated that VEGF-D closely related to invasion and lymphatic metastasis of hepatocellular carcinoma.2 Numerical value of flt-4 positive number was higher in tumor tissue than in para-carcinoma tissues and normal tissue, this suggested that high flt-4 positive number value is one of the characters of hepatocellular carcinoma and it has a very important role in invasive and lymphatic metastasis progression in hepatocellular carcinoma. The results indicate that VEGF-D can accelerate lymphatic angiogenesis in hepatocellular carcinoma. It may be correlated with invasion and lymphatic metastasis of hepatocellular carcinoma.3 There is closely relationship between the expression of VEGF-D and flt-4 in pancreatic cancer. VEGF-D and flt-4 may be valuable indicators to prognosis, monitoring recurrence and lymphatic metastasis, assessing therapeutic effect in hepatocellular carcinoma.
|
PDF Full Text Request