Study On The Relationship Between The Levels Of Calcium, Magnesium In Serum And Airway Inflammation In Patients With Chronic Obstructive Pulmonary Disease

Objectives Part One: To research the relations between lower respiratory infection(LRI) with hypocalcemia patients and infected frequence, and the relations betweenchronic obstructive pulmonary disease (COPD)with hypocalcemia patients andinfected frequence were also researched in the study. Part Two: To observe thelevels of calcium, phosphorus, magnesium in serum and 24 hours' urine Ca,P, Mgand I1-8,IL-13 in serum of acute chronic obstructive pulmonary disease (COPD) andstable COPD patients, and to discuss the relationship between serum Ca, P,Mg,cytokines and airway Inflammation in the study.Methods Part One: The subjects were 126 LRI patients (COPD patients 78) fromthe Total Hospital of Tianjin Medical University inpatients, and to investigate thelevel of serum calcium and infected frequence in recent tow years. Part Two:Subjects were 30 patients with COPD to be parted as acute stage group and stablestage group and 30 healthy individuals from medical examination center (MEC). Allof them were evaluated by thorough medical history and complete physicalexamination, which may exclude diseases to ensure the results true. In order tostudy the correlativities among them, the contents of serum Ca and 24 hours' urineCa were measured with endpoint colorimetric assay, serum P and 24 hours' urine Pwere measured with ultraviolet spectrophotometry, serum Mg and 24 hours' urineMg were measured with xylidine blue assay, serum ALP were measured with SFBCvelocity method, all of them were measured with reagent of Roche company byHitachi 7170A entire automatic biochemistry analyzer, and the levels of IL-8, IL-13in serum were measured with enzyme linked immunosorbent assay, as well as thelevels of pulmonary function (FEV1, FVC, FEV1/FVC) and blood gas analysiswere measured.Results Part One:â‘ There was significant deviation in infected frequence between LRI with hypocalcemia patients and non-hypocalcemia patients(x²=51.315, p＜0.01);â‘¡There was also significant deviation in infected frequencebetween COPD with hypocalcemia patients and non-hypocalcemia patients(x²=43.296, p＜0.01). Part Two:â‘ The levels of serum Ca, Mg in acute COPDpatients were decreased obviously, which compared to healthy control group(p＜0.05,p＜0.05); The levels of serum Ca, Mg in stable COPD patients were decreasedobviously, which compared to healthy control group (p＜0.05, p＜0.05); There wasdeviation of serum ALP between the different groups of COPD patients and healthyindividual, but not statistically significant (p＞0.05). The levels of Ca, P, Mg in 24hours' urine in acute and stable COPD patients were lower than normal referencevalue; There was not significant deviation of serum Ca, P, Mg and in 24 hours' urineCa, P, Mg between acute and stable COPD patients.â‘¡The levels of serum IL-8,IL-13 in acute COPD patients were increased remarkably, which compared tohealthy control group (p＜0.05, p＜0.05); The levels of serum IL-8, IL-13 in stableCOPD patients were increased remarkably, which compared to healthy controlgroup (p＜0.05, p＜0.05), but not statistically significant between acute and stablestage COPD patients (p＞0.05).â‘¢There was significant negative correlationbetween serum IL-8, IL-13 and serum Ca in acute COPD patients (r=-0.484,p＜0.01; r=-0.502, p＜0.01); There was significant negative correlation betweenserum IL-8, IL-13 and serum Mg in acute COPD patients (r=-0.492, p＜0.01; r=-0.552, p＜0.01).â‘£There was significant positive correlation between serum Caand FEV1, FVC, FEV1/FVC(r=0.364, p＜0.05; r=0.454, p＜0.05; r=0.452,p＜0.05); There was significant positive correlation between serum Mg and FEV1,FVC, FEV1/FVC (r=0.387,p＜0.05; r=0.434,p＜0.05; r=0.476,p＜0.01).⑤Therewas significant negative correlation between serum IL-8 and FEV1, FVC,FEV1/FVC (r=-0.600,p＜0.01; r=-0.607,p＜0.01;r=-0.610,p＜0.01); There wassignificant negative correlation between serum IL-13 and FEV1/FVC (r=-0.453,p＜0.05); There didn't have correlation between serum Mg and FEV1, FVC.â‘¥ There was significant negative correlation between blood gas analysis PH value andthe level of P in 24 hours' urine (r=-0.461, p＜0.05); There was significant positivecorrelation between PaCO₂ and the level of P in 24 hours' urine (r=0.493, p＜0.01);There was significant positive correlation between PaO₂ and the level of P in 24hours'urine (r=0.411, p＜0.05).Conclusions Part One:â‘ The repeated infected frequence of LRI withhypocalcemia patients were increaser obviously than non-hypocalcemia patients.â‘¡The repeated infected frequence of COPD with hypocalcemia patients wereincreaser obviously than non-hypocalcemia patients.â‘¢Therefore, according toexperimental result, as if COPD with hypocalcemia patients were easier to takeplace respiration infection than non-hypocalcemia patients, we will consider to treatwith Vitamin D or Suplical, it maybe boost organism immune competence. So it hassignificant role for nonage precaution, diagnosis and treatment, thus it can stepdown COPD advancement and decrease complication of COPD.Part Two:â‘ The levels of serum Ca, Mg whether in acute stage or stable stageCOPD patients were lower than healthy adults. The levels of serum IL-8, IL-13 indifferent stage COPD patients were super than healthy adults.â‘¡There was closerelationship between serum Ca, Mg and cytokines.There was significant negativecorrelation between serum IL-8, IL-13 and serum Ca,Mg in acute COPD patients;There was significant positive correlation between serum Ca,Mg and pulmonaryfunction.â‘¢Therefore, according to experimental result, There was closerelationship between serum Ca, Mg and airway inflammation. It make damage ofpulmonary function more serious. So Ca, P and Mg element bring into important roleon regulating cytoactive, enzymatic function and organism immunity. They cancontrol the externalization and activity of cytokine directly or indirectly. At a someextent, they may reflect degree of COPD patient's condition.