Study On The Serum Levels Of TGF-β₁,IL-13,IL-8 Of Patients With Idiopathic Pulmonary Fibrosis

Objective The aim of this study isâ‘ to detect the levels of transforming growthfactor-betal (TGF-β₁), interleukin-13 (IL-13), interleukin-8(IL-8) in the serum ofpatients with idiopathic pulmonary fibrosis (IPF). To explore the role of the threecytokines in the pathogenesis of pulmonary fibrosis.â‘¡To analyze the correlation ofthe level ofTGF-β₁,IL-13,IL-8 of the serum and the pulmonary function tests andthe arterial blood gases of the IPF patients, to investigate the clinical significance ofthe three cytokines in the evaluating the severity and the progression of the disease.Methods The study population consisted of 26 patients with IPF, and 30healthy controls. We measured the concentrations of TGF-β₁,IL-13,IL-8 in theserum of all the participants by ELISA, We also measured the concentration of LDHof all the patients. We obtained the arterial blood gases parameters partial pressure ofoxygen in arterial blood(PaO₂), partial pressure of carbon dioxide in arterialblood(PaCO₂), arterial oxygen saturation (SaO₂), partial pressure of alveolar- arterialoxygen difference (P_(A-a)O₂), and pulmonary function tests parameters forced vitalcapacity (FVC), forced expiratory volume in 1 second (FEV₁), and forced expiratoryvolume in the frist second/forced vital capacity (FEV₁/FVC) from all the patients.Resultsâ‘ The concentrations of TGF-β₁ were significantly higher in IPFpatients (743.43±172.62 pg/ml) than in controls (287.44±66.54 pg/ml)(p＜0.01).â‘¡The concentrations of IL-13 were significantly higher in IPF patients (26.93±1.90pg/ml) than in controls(13.71±0.84 pg/ml pg/ml) (p＜0.01).â‘¢The concentrations ofIL-8 were significantly higher in IPF patients (233.30±23.91pg/ml) than incontrols(186.87±22.97 pg/ml)(p＜0.01).â‘£The concentrations of TGF-β₁ negativelycorrelated to the PaO2(r=-0.389, p＜0.05), while positively correlated to theP(A-a)O2 (r=0.442, p＜0.05); negatively correlated to the FVC(r=-0.541, p＜0.01), negatively correlated to the FEV₁(r=-0.465, p＜0.05).⑤The concentrations of IL-13positively correlated to the P_(A-a)O₂ (r=0.525, p＜0.01); negatively correlated to theFEV₁(r=-0.453, p＜0.05).â‘¥The concentrations of IL-8 negatively correlated tothe PaO₂(r=-0.542, p＜0.01), negatively correlated to the SaO₂ (r=-0.526, p＜0.05);negatively correlated to the FVC(r=-0.429, p＜0.05).Conculsionâ‘ The concentrations of TGF-β₁,IL-13,IL-8 were significantlyhigher in IPF patients than in controls, it suggests that the three cytokines may playimportant roles in the pathogenesis of pulmonary fibrosis.â‘¡There were correlationbetween the concentrations of TGF-β₁,IL-13,IL-8 of serum and the arterial bloodgases parameters and the pulmonary function tests parameter in IPF patients. With theincreasing of the serumal concentrations of TGF-β₁,IL-13,IL-8 in IPF patients, thearterial blood gases parameters PaO₂ and SaO₂ decreased, P_(A-a)O₂ increased, thepulmonary function parameters FVC and FEV₁ decreased. It suggests theconcentrations of TGF-β₁,IL-13,IL-8 in the serum of IPF patients may be usefulmarkers in the evaluating the severity and progression of the disease.