| Objective:Osteoporosis is one of the most common metabolic bone diseases. Over half ofdiabetes mellitus (DM) patients have osteoporosis (OP). During the length of lifetimeper capita, diabetic osteoporosis that has the features of higher incidence rate, highermortality rate, higher mutilation and lesser life quality has been a troublesomeproblem for public health and social life. Xylitol that can promote insulin secretionand inhibit ketoplasia, as the succedaneum of cane sugar, is currently considered themain sweet additives of the foods of DM patients. There are animal experimentsproving that Xylitol can facilitate the early bone mineralization with energizing forcalcium absorption, augment the formation of the proline by holding back itsdegradation by suppressing collagen catabolic enzymes activity, and make lessdamage to osteoblast for both glycosylation and oxidation. To conclude, Xylitol maybe a new type of measure to osteoporosis treatment, then protect against weaking ofbone density, bone histomorphlolgy and bone biomechanical properties in DM. Weevaluated the therapeutic effects of Xylitol on osteoporosis of DM rats, finding themechanism of action and dose response relation.Methods:Fifty four male Wistar rats of three months old were randomly divided into sixgroups: DM control group, DM+10%Xylitol group, DM+20%Xylitol group, Normalcontrol group, Normal+10%Xylitol group, normal+20%Xylitol group. DC group andNC group were fed a basic diet; DM+10%Xylitol group, DM+20%Xylitol group,Normal+10%Xylitol group, normal+20%Xylitol group were fed the same dietsupplemented with Xylitol, it's concertration in the grain of 10% and 20%(wt/wt).After 12 weeks, the rats were killed by decapitation, keeping the blood for theanalysis of biochemical indicators, the bone for the analysis of bone mineral density(BMD), the biomechanical properties and morphological parameters. Results:(1) Serum Glucose:①At 0 week after treatment between groups: comparing with NC group, the serumglucose concentration of DC group significantly increased, the difference hadstatistical significance(P＜0.001). At 5 week: comparing with NC group, the serumglucose concentration of DC group significantly increased, the difference hadstatistical significance(P＜0.001); Comparing with DC group, the serum glucoseconcentration of DM+20%Xy group degraded, the difference had statisticalsignificance(P＜0.05). Comparing with DM+10%Xy group, the serum glucoseconcentration of DM+20%Xy group lowered, the difference had statisticalsignificance(P＜0.05). At 12 week: comparing with NC group, the serum glucoseconcentration of DC group significantly increased, the difference had statisticalsignificance(P＜0.001). Comparing with DC group, the serum glucoseconcentration of DM+10%Xy group degraded, the difference had statisticalsignificance(P＜0.05). Comparing with DC group, the serum glucoseconcentration of DM+20%Xy group lowered, the difference had statisticalsignificance(P＜0.05).②Comparing with 0 week, the serum glucose concentration of all groups in 5 week,the difference had not statistical significance(P＞0.05). Comparing with 0 week,the serum glucose concentration of DM+20%Xy group in 12 week cut down, thedifference had statistical significance(P＜0.01). Comparing with 5 week, the serumglucose concentration of DM+10%Xy group in 12 week cut down, the differencehad statistical significance(P＜0.05). Comparing with 5 week, the serum glucoseconcentration of DM+20%Xy group in 12 week cut down, the difference hadstatistical significance(P＜0.05).(2) Body weight between at 0 week after treatment: there were not different of thebody weights of all groups (F=19.353, P＞0.05). At 12 week: comparing withNC group, the body weight of DC group degraded, the difference had statisticalsignificance (P＜0.01). Comparing with DC group, the body weight of DM+20%Xy group advanced, the difference had statistical significance (P＜0.05).(3) Serum calcium between groups at 12 week after treatment: there were notdifferent of all groups(F=2.006, P＞0.05). Serum phosphonium: there were notdifferent of all groups(F=2.064, P＞0.05).Serum ALP: comparing with NC group,the serum ALP of DC group degraded, the difference had statistical significance(P＜0.001). Serum BGP: comparing with NC group, the serum BGP of DC groupdegraded, the difference had statistical significance (P＜0.05).(4) BMD between groups at 12 week after treatment: comparing with NC group,BMD of DC group degraded, the difference had statistical significance (P＜0.001).Comparing with DC group, BMD of DM+20%Xy group advanced, the differencehad statistical significance (P＜0.001). Comparing with NC group, BMD ofN+20%Xy group advanced, the difference had statistical significance (P＜0.05).Comparing with N+10%Xy group, BMD of N+20%Xy group advanced, thedifference had statistical significance (P＜0.05). The results of right femur weresame with left.(5) Bone morphometry parameters between groups at 12 week after treatment:①Comparing with DC group, MTPD of DM+20%Xy group advanced, the differencehad statistical significance (P＜0.05). Comparing with DM+10%Xy group, MTPDof DM+20%Xy group advanced, the difference had statistical significance(P＜0.05).②Comparing with NC group, MTPS of DC group advanced, the difference hadstatistical significance (P＜0.05).③Comparing with NC group, DDL of DC group degraded, the difference hadstatistical significance (P＜0.01). Comparing with DC group, DDL ofDM+20%Xy group advanced, the difference had statistical significance (P＜0.01).④Comparing with NC group, MiAR of DC group degraded, the difference hadstatistical significance (P＜0.01). Comparing with DC group, MiAR ofDM+20%Xy group advanced, the difference had statistical significance (P＜0.01).⑤Comparing with NC group, Svf of DC group degraded, the difference had statistical significance (P＜0.01). Comparing with DC group, Svf of DM+20%Xygroup advanced, the difference had statistical significance (P＜0.01).(6) The index comparison in the compressive test of the femur metaphysis betweengroups at 12 week after treatment:①Comparing with NC group, elasticity load of DC group degraded, the differencehad statistical significance (P＜0.01). Comparing with DC group, elasticity load ofDM+20%Xy group advanced, the difference had statistical significance (P＜0.05).②Comparing with NC group, the maximum load of DC group degraded, thedifference had statistical significance (P＜0.01).③Comparing with NC group, proportional limit of DC group degraded, thedifference had statistical significance (P＜0.01). Comparing with DC group,proportional limit of DM+20%Xy group advanced, the difference had statisticalsignificance (P＜0.05).④Comparing with NC group, crushing strength of DC group degraded, thedifference had statistical significance (P＜0.01).⑤Comparing with NC group, elastic modulus of DC group degraded, the differencehad statistical significance (P＜0.01).(7) The index comparison in the tree-point bending test of the tibia between groups at12 week after treatment:①Comparing with DC group, elasticity flexibility of DM+10%Xy group advanced,the difference had statistical significance (P＜0.01). Comparing with DC group,elasticity flexibility of DM+20%Xy group advanced, the difference had statisticalsignificance (P＜0.05).②Comparing with NC group, elasticity load of DC group degraded, the differencehad statistical significance (P＜0.001). Comparing with DC group, proportionallimit of DM+20%Xy group advanced, the difference had statistical significance(p＜0.05).③Comparing with NC group, maximum flexibility of DC group degraded, thedifference had statistical significance (P＜0.01). ④Comparing with NC group, Youning's modulus of DC group degraded, thedifference had statistical significance (P＜0.01).⑤Comparing with NC group, bending energy of DC group degraded, the differencehad statistical significance (P＜0.01). Comparing with DC group, bending energyof DM+20%Xy group advanced, the difference had statistical significance(P＜0.05). Comparing with DM+10%Xy group, bending energy of DM+20%Xygroup advanced, the difference had statistical significance (P＜0.01).⑥Comparing with NC group, bending rigidity coefficient of DC group degraded, thedifference had statistical significance (P＜0.01). Comparing with DC group,bending rigidity coefficient of DM+20%Xy group advanced, the difference hadstatistical significance (P＜0.05). Comparing with DM+10%Xy group, bendingrigidity coefficient of DM+20%Xy group advanced, the difference had statisticalsignificance (P＜0.01).Conclusion:These results showed that (1) the decrease of weight, BMD, bone biomechanicalproperties and histomorphometric parameters, increase of glucose in DM rats,(2)Dietary Xylitol Supplementation prevented Osteoporotic changes in Diabetes Rats.The effect in high dose was obvious, being present dose-dependent. (3) Xylitol hadnot effects on the glucose of health rats, but could increase bone mineral content andbone density. |
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