HPV Infection And TAP1 Polymorphism In Kazakh Esophageal Squamous Cell Carcinomas From Xinjiang

Objective: (1) Evaluate the prevalence of HPV infection in Kazakh esophageal squamous cell carcinoma(ESCC)of Xinjiang.(2)To investigate correlations of the polymorphisms at TAP1 genes (Transporter associated with antigen processing), with the risk of Kazakh ESCC in Xinjiang. Analyze the combined effect of TAP1 and HPV infection on ESCC in Kazakh in Xinjiang.Methods: (1)Polymerase chain reaction(PCR) was employed to screen HPV infection statuses of all samples based on five pairs of primers(general primers GP5+/6+ for HPV L1, type-specific primer for HPV-16,18,31,45.(2)TAP1 Codon333 and Codon637 genotypes were detected by a PCR-based RFLP assay in 150 cases with esophageal carcinoma and 283 cases cancer-free controls in the same area. (3)Theχ2 test was adopted to check the difference in genotype distribution between cases and controls, and the accordance with Hardy-Weinberg equilibrium in the group.Results:(1) The general infection rates, infection rates of HPV-16,18,31,45E7 and multi-infection statuses were analyzed between ESCC and"normal"esophageal carcinoma(NE), the results were as follows: the general infection rates, infection rates of HPV-16,18,31 type and multi-infection statuses , were significantly higher in ESCC than those in NE﹙P<0.05﹚, the infection rates were 64.6%,41.1%,25.3%,14.2%, 7.3% and 4.5%,18.2%, 15.2%, 9.1%, 4.5%,1.5%and 4.5%,respectively., The OR values of HPV-16, 18 and multi-infection statuses were 8.196,3.941, 3.390 and 4.523 , respectively;. (2) The frequencies of AA, AG and GG in TAP1 Codon637 were 48.6%,46.7% and 4.7% in Kazakh's individuals with EC, and 22.8%,52.6%and 24.6% in Kazakh's controls, respectively, a significant difference between frequencies of A/A ,A/G and G/Gwas found(P <0.05). The genotype with G allele improved the odds ratio for individuals with EC 1.659.(3)The frequencies of AA, AG and GG in TAP1 Codon333 were 54.7%,41.3%,and 4.0% in Kazakh's individuals with EC, respectively. They were not significantly different from that in Kazakh's controls (47.3%,46.0% and 6.7%). (4).The genotype frequency of TAP1Codon637 polymorphisms, there is significant difference between HPV positive cases and HPV negative cases, P <0.05. however, for the three genotpyes of TAP1Codon333, there is no significant difference between HPV positive cases and HPV negative cases. Conclusion: (1). HPV DNA infection had relationship in Kazakh ESCC of Xinjiang. HPV-16, 18 were the most common types, HPV infection may be one of risk factor in Kazakh ESCC. (2). There may be one of genetic susceptibility factors that TAP1 Codon637 polymorphism of EC in Kazakh in Xinjiang. There maybe no relationship between the TAP1 Codon333 polymorphism and risk of Kazakh esophageal carcinoma in Xinjiang. (3). TAP1 Codon637 polymorphism and HPV infection may be associated with Kazakh esophageal cancer in Xinjiang.