A Study Of Effect On Calcium Oxalate Nephrolithiasis By Lactobacillus In Stone Forming Rats.

Objective The aim of this study was to evaluate whether oral administration of lactobacilli could prevent calcium oxalate nephrolithiasis in stone-forming rats.Methods Calcium oxalate stone-forming rats were induced by ammonium chloride and ethylene glycol dissolved in drinking water. The rats were randomly divided into 3 groups: (n=10, each). (1)controlling rats, (2)stone-forming rats, (3)oral administration of lactobacilli on stone-forming rats. On days 28 and 29 of the experiment, rats were transferred to metabolic cages for 24-h urine collection and then sacrificed. Urine volume and pH was determined using freshly voided urine. Concentrations of urinary oxalate, citrate, calcium were determined using a chemical method. The creatinine clearance was measured for evaluating renal function. The excised kidney was quickly frozen, fixed with formalin and embedded with paraffin then made of slice up for examined under a dissecting microscope for crystal numbers and distribution.Results No statistical differences were found in terms of urine volume and creatinine clearance between groups. Urinary pH level was lower in stone-forming rats than the other two groups, but this was not significantly different. Concentration of citrate was higher in lactobacillus-treated rats group and stone-forming rats group than control group, there were significantly different in the mean levels of urinary excretion of citrate between the lactobacillus-treated rats group versus the control group and the stone-forming rats group versus the control group, respectively. The calcium level in lactobacillus-treated rats group and stone-forming rats group was lower than the control group, there were also significant differences in the mean levels of urinary excretion of calcium between the lactobacillus-treated rats group versus the control group and the stone-forming rats group versus the control group, respectively. Because of physiologic results of calcium oxalate crystal formation. Concentration of oxalate in group of stone-forming rats was significantly elevated versus the control. Concentration of oxalate in lactobacillus-treated rats was lower than stone-forming group. Significant differences in levels were observed between groups.Conclusions lactobacilli may used as a potential therapeutic strategy in the prevention of calcium oxalate nephrolithiasis.