The Experimental Study About Inhibitory Effects Of Xiao Kang Ling â…¡ On Uu In Vitro

Background :Ureaplasma urealyticum(Uu) is a kind of microorganism with no cell wall.Uu can survive asymptomaic in reproductive and urinary tract for a long time.It can cause kinds of disease. For example. vaginitis.cervicitis.PID.infertility.etc.My tutor professor Han invent Xiao Kangling II though clinic experience to inhibit the growth of Uu and enhance the immunity .Objective:The experiment trys to study the mechanism of Xiao Kangling II inhibiting the growth of Uu and testify its superority to AZI.Then.simplfy the composition of Xiao KangLing II.Methods:By means of inhibitory test in vitro. This experiment uses double dilution to dilute the Chinese medicine herbs and AZI to ten concentration.After 24～48h.we observe the result.Result: CD The inhibitory effect of solo Chinese medicine herb:Yu xingcao (MIC₅₀: 7.81g/l.MIC₉₀: 62.5 g/l. Sensitive rate: 80.00%) .Chui pencao ( MIC₅₀: 7.81 g/l.MIC₉₀: 62.5g/l.Sensitive rate: 70.00 %) . Huang bai (MIC₅₀: 7.81 g/l. MIC₉₀: 62.5g/l. Sensitive rate: 53.33 %) .Lian qiao (MIC₅₀:7.81 g/l.MIC₉₀: 125.00 g/l.Sensitive rate: 53.33%) .Huang qi ( MIC₅₀: 3 1.25 g/l.MIC₉₀: 250.00 g/l. sensitive rate:23.33 %) .Gan cao ( MIC₅₀: 31.25 g/l.MIC₉₀: 250.00 g/l. Sensitive rate: 16.67 %) .Tu fuling (MIC₅₀: 31.25 g/l.MIC₉₀: 125.00 g/l. Sensitive rate:10.00%) .Dang shen (MIC₅₀: 62.5 g/l.MIC₉₀: 250.00 g/l. Sensitive rate: 0) .â‘¡The inhibitory effect of Xiao kangling II and AZI: Xiao kangling II:MIC₅₀: 7.81g/l. MIC₉₀:62.5 g/l. Sensitive rate: 73.33%, Drug resistant strains:0. AZI:MIC₅₀:0.078g/l.MIC₉₀:1.25g/l. Sensitive rate:53.33%. Drug resistant strains : 8 (26.67%) .â‘¢Simplified Xiao kang ling II :MIC₅₀:7.81 g/l.MIC₉₀:31.25 g/l. Sensitive rate:83.33%.Drug resistant strains:0.Conclusion:â‘ Xiao kangling II have a clear inhibitory effect to Uu.After simplifying the composition.the MIC is unconverted. But. The sensitivity rate elevates.â‘¡AZI have also a clear inhibitory effect to Uu.but.there are many drug-resistant strains.