Experimental Study Of Transplantation Of Human Mesenchymal Stem Cells In Treatment Of Experimental Allergic Encephalomyelitis In Rats

Objective: To study the effect of transplantation of the human mesenchymal stem cells(hMSCs) in treatment of experimental allergic encephalomyelitis(EAE) rats and the status of transplanted hMSCs in the brain tissue of EAE rats.Materials and Methods: hMSCs were isolated from human adult bone marrow. EAE rat models were made ,and the human mesenchymal stem cells were transplanted into the rat through tail vein 10,15 and 21 days after immunization.The neurological signs were undertaken in all groups every day after immunization.Rats were sacrificed at different time.The differentiation and migration of the human mesenchymal stem cells in vivo, and the number and status of the demyelinated loci were checked by electronic microscope and immunohistology. The expression of spinal cord MBP mRNA and PDGFα-R mRNA were detected by semiquantita- tive reverse transcriptase polymerase chain reaction(RT-PCR). Results: Rats that received transplantation had a significant improvement compared with the control group in the observation of Electronic microscope. Twenty days later, rats that received transplantation 10 and 15 days after immunization had a significant improvement of clinical outcome compared with the control group (P＜0.05). The number of demyelinated loci decreased significantly(P＜0.05). The human mesenchymal stem cells were induced to differentiate into astrocytes, oligodendrocytes and neurons in vivo. Immunohistological result confirmed that the transplanted stem cells migrated to the demyelinated foci. In compareson with control group, the expression of spinal cord MBP mRNA and PDGFα-R mRNA in transplantation group were significantly higher and then up-regulated gradually.Conclusions: The human mesenchymal stem cells differentiated into three main types of neural cells by the influcence of the EAE microenvironment signals.Human mesenchymal stem cells transplantation could effectively improve the neurologic function of rats,and decrease demyelinated loci number. Objective: To study the state of cortex neuron apoptosis after transplantation of human mesenchymal stem cells(hMSCs) in experimental allergic encephalomyelitis(EAE) rats.Materials and Methods: hMSCs were isolated from human adult bone marrow. EAE rat models were established and the hMSCs were transplanted into the rat tail vein 10 days after immunization to observe the influence of hMSCs to cortex neuron apoptosis. Immunohistological method was used to see the survival, migration and differentiation of hMSCs in EAE frontal cortex.Results: The mesenchymal stem cells were cultured successfully. Brdu and NSE positive cells could be present in almost all frontal cortex sections at every time point.The apoptosis of cortex neuron decreased significantly in hMSCs transplantation group (P＜0.05). Brdu and NSE positive cells of 10 day and 30 day after transplantation increased significantly than 2 day after transplantation(P＜0.05). Conclusions: Apoptosis existed in frontal cortex of EAE rats; hMSCs have multipotential differentiation properties in vivo and in vitro,the transplanted cells could migrate to EAE cortex and differentiate into NSE positive neurons to replace apoptosis cells.