Paraoxonase/Glutathione S-ransferase Gene Polymorphism And Health Conditions Of Floriculture Farmers

Objective : To investigate Paraoxonase and Glutathione S-ransferase GenePolymorphism in floriculture, and assess the relationship between gene polymorphismand health condition in floriculturists.Methods: 136 pesticide-exposed farmers and 41 local non-pesticide-direct exposedlocals as control. PON1 and GSTM1/T1 genotype were determined by mean ofPolymerase Chain Reaction-Restriction Fragment lengty Polymorphism (PCR-RFLP).Assess if there were associations between genotype and AChE activity,Immunefunction,Endocrinosity,Clinical health index,symptoms.Result:1,PON1 and healthIn all 177 cases, PON1 regulatory-region-107 and coding region 192 and 55 were polymorphisms.There have no accociation between genotype/alleles frequence and sex.(1) PON1-107CT genotype is associated with globin in high exposure group (P<0．05) . Compare same genotype CT in different groups, IgG were significant lower in high and low exposure group than in control group(P＜0．01, P＜0．05) ; Globin were significant lower in high and low exposure group than in control group(P＜0．05, P＜0．05) ; A/G were significant higher in high and low exposure group than in control group(P＜0．01, P＜0．05) ; T was significant lower in high exposure group than in low exposure group(P<0．05) .(2) PON1 192According to different genotypes: ALT in Arg/Arg genotype was higher in Gln/Gln genotype(P<0．05) ; AST in Arg/Arg and Gln/Arg genotype were higher in Gln/Gln genotype(P<0．01,P＜0．05) ; T₄ in Arg/Arg genotype was higher in Gln/Arg genotype(P<0．05) ; T₄ Arg/Arg genotype was higher Gln/Arg in high exposure group(P<0．05) ; T Gln/Arg genotype in high exposure group was higher than in control group(P<0．05) .(3) PON1 55There were no associations between genotype and health index.2,GSTM1/T1 and health(1) GSTM1There were no associations observed between genotype and health index.(2) GSTT1T GSTT1+ genotype was higher than GSTT1-(P<0．01) ; TP GSTT1+ genotype was lower than GSTT1-(P＜0．01) ; GB GSTT1+ genotype was lower than GSTT1-(P<0．01) ; A/G GSTT1+ genotype was higher than GSTT1-(P<0．01) ; IgG GSTT1+ genotype was lower than GSTT1-(P＜0．05) ; AST,TP,GB GSTT1+ genotype was lower than GSTT1-in high exposure group(P<0．05) ; A/G GSTT1+ genotype was higher than GSTT1-in high exposure group(P<0．05) ; IgA GSTT1+ genotype was higher than GSTT1-in high exposure group(P<0．05) .(3) GSTM1 and GSTT1Platet GSTM1+/GSTT1+ genotype was higher than GSTM1-/GSTT1-(P＜0．05) .3 Gene polymorphism and logistic regressionThe younger the age, The more the symptoms; The persons with GSTM1+, the symptoms were 2．663 times than the persons with GSTM1-.Conclusion:(1) PON1 107 was a possible susceptibility biomarker the harm of pesticide to immune system;(2) PON1 192 was a possible susceptibility biomarker the harm of pesticide to endocrinosity;(3) There were no association between PON1 55 genotype and health index;(4) The symptoms in GSTM1+ genotype were higher in GSTM1-genotype;(5) GSTT1 was a possible susceptibility biomarker the harm of pesticide to immune and endocrinosity system.