Study On The Constituents Of Tabanus Bivittatus Mats. Ⅰ

Tabanus bivittatus Mats. is a species which comes from Tabanidae of Diptera .The dried body of T. bivittatus has been used as a Traditional Chinese Medicine. A lot of studies on the activity of polypeptide and polysaccharide from the extraction have been carried out. However, there is few report about the chemical constituents from the dried body of T. bivittatus.Firstly, the anticoagulation and antiinflammatory activities of the extractions from T. bivittatus were detected. The result showed that the anticoagulation activity of the extractions was not obtained. Otherwise, the extractions significantly inhibited ear-swelling in xylene-treated mice (p＜0.01).In this paper, the chemical constituents from EtOAc and Pet Eth fraction of the 70% ethanol extraction were investigated. Fourteen compounds were isolated from EtOAC and Pet Eth fraction by repeated silica gel column chromatography, Sephadex LH-20, TLC, RP-HPLC, and so on. The structures of ten compounds were identified on the basis of their physico-chemical properties, spectroscopic analysis, and the data from literatures. The compounds are shown as follows: cholestenol (TE-01), bis (2-ethylhexyl) phthalate (TE-02), 3-methoxy-methylbenzoic acid (TE-03), cytosine (TE-04), 4-(2-(methylthio)ethyl)phenol (TE-05), uracil (TE-06), N-(2-(3,4-dihydroxyphenyl)-2-methl-oxyethyl)acetamide (TE-07), cholesterol (TE-10), Thymine (TE-11), 2-(2-hydroxyphenyl)-2-methoxy-ethylcarbamic acid (TE-14). Compound TE-07 was isolated from nature for the first time and compound TE-02 was isolated from animal for the first time.Meanwhile, the lipid-soluble constituents from the dried body of T. bivittatus were studied by GC-MS and twenty compounds were identified.