The Experimental Research Of Human Umbilical Cord-derived Mesenchymal Stem Cells (MSCs) Differentiated Into Insulin-producing Cells

Objective:To explore the possibility of inducing human umbilical cord Wharton's Jelly-derived mesenchymal stem cells (MSCs) by nicotimine andβ-mercaptoethanol to differentiate into insulin-producing cells; to provide an important insight and a new resource of celltransplant to cure Type 1 diabetes mellitus(T1DM).Materials and methods:Mesenchymal stem cells (MSCs) were cultured from the Wharton's Jelly taken from full-term section birth. After Preinduced by L-DMEM with nicotimine andβ-mercaptoethanol, mesenchymal stem cells(MSCs) were induced by H-DMEM within nicotimine andβ-mercaptoethanol. The expression of insulin marker was shown by immunocytochemistry, Zincum in cells was dyed by dithizone, and the levels of insulin in the media were detected by radioimmunoasay(RIA).Results:Mesenchymal stem cells(MSCs) clutured from human umbilical cord Wharton's Jelly were processed to obtain a fibroblast-like population of cells. They could be maintained in vitro for extended periods with stable population doubling, and they were expanded as undifferentiated cells in cultrue for more than 10 passages, indicating their proliferative capacity. Association of nicotimine andβ-mercaptoethanol could induce mesenchymal stem cells(MSCs) differentiated to insulin producing cells. Insulin, the marker ofβcells of islet, was expressed in the differentiated cells. Dying by dithizone showed us zincum in those cells derived from human umbilical cord Wharton's Jelly had an important increase when they were induced by nicotimine andβ-mercaptoethanol. Radioimmunoasay of insulin showed that the levels of insulin in experimental group and control group were 6.63mIU/L and 3.39mIU/L, and the difference had statistical significance(p＜0.05). Conclusion:The human mesenchymal stem cells(MSCs) could be isolted from umbilical cord Wharton's Jelly, and could maintain good puripotentiality and self-renewal capacity. Mesenchymal stem cells(MSCs) derived from human umbilical cord Whwrton's Jelly expressed the marker ofβcell of islet after induced by nicotimine andβ-mercaptoethanol. And the level of zincum in the differentiated cells were much increased than it was in the undifferentiated cells. Much more, insulin was detected in the media of the defferentiated cells. This study showed that nicotimine andβ-mercaptoethanol could induce the mesenchymal stem cells(MSCs) into insulin producing cells, thereby provided a new rescource of cell transplantion to cure Type 1 diabetes mellitus(T1DM).