Study And Application Of Flow Injection Chemiluminescence On The Pharmaceuticals Analysis

Chemiluminescence (CL) analysis is one of trace analytical methods, which is based on the light emission during the course of some chemical reactions. The coupling of flow injection analysis (FIA) with CL detection has been rapidly developed, due to a serial of advantages such as simple instrument, rapid analysis, high sensitivity, wide linear range, good repeatability, and easy automatization. It is not only of great theoretical significence but also of application value that chemiluminescence is applied in the pharmaceutical analysis.The mechanism of CL and the basic theory of FIA were introduced systemically and an overview on the combination of CL with flow injection was presented in the pharmaceutical analysis since 1996 in this paper. And discussion about the following points has been held.1. A novel chemiluminescence method for rapid determination of shikimic acid with flow injection analysis was presented in this paper. It based on the CL reaction of shikimic acid and potassium permanganate in sulfuric acid medium. The linear relationship between the chemiluminescence intensity and the concentration of shikimic acid is over the range of 2.0×10^-6 - 1.0×10^-3mol/L. The detection limit is 7.2×10^-7 mol/L and the RSD is 1.4% for 1.0×10^-4mol/L shikimic acid (n=7). The CL system is simple, stable and has wide linear range. The recommended method was successfully applied to the determination of shikimic acid in illicium vemm hook.f..2. A new flow injection chemiluminescence method for the determination of penicillamine was establishe. The method was based on chemiluminescence reaction of hydrogen peroxide with sodium periodate in phosphoric acid condition enhanced by penicillamine. The method is simple, convenient and sensitive. The linear relationship between the intensity of chemiluminescence and the concentration of penicillamine is over the range of 1.0×10^-6 - 8.0×10^-4 mol/L. The detection limit is 5.6×10^-7 mol/L and the RSD is 1.9% for 8.0×10^-5 mol/L penicillamine (n=7). This method has been successfully applied to the determination of penicillamine in tablets with recovery between 97.8% and 104.4%. The mechanism of the reaction was also discussed.3. A new flow injection chemiluminescence method for the determination of rutin was established. The method was based on chemiluminescence reaction of rutin with Cerium(IV) sulfate in sulfuric acid condition sensitized by rhodamine 6G. The method is simple, convenient and sensitive. The linear relationship between the intensity of chemiluminescence and the concentration of rutin is over the range of 1.0×10^-7 - 1.0×10^-4 mol/L. The detection limit is 8.1×10^-8 mol/L and the RSD is 1.5% for 1.0×10^-5mol/L rutin (n=7). This method has been successfully applied to the determination of rutin in tablets with recovery between 99.0% and 104.0%.4. A new flow injection chemiluminescence method for the determination of paeonol was established. The method was based on chemiluminescence reaction of paeonol with Cerium(IV) sulfate in sulfuric acid condition sensitized by rhodamine 6G. The method is simple, convenient and sensitive. The linear relationship between the intensity of chemiluminescence and the concentration of paeonol is over the range of 5.0×10^-7 - 1.0×10^-4mol/L. The detection limit is 9.7×10^-8 mol/L and the RSD is 1.8 % for 1.0×10^-5mol/L paeonol (n=7). This method has been successfully applied to the determination of paeonol in ointment with recovery between 98.8% and 104%, and the results were in good agreement with those obtained by UV method.