The Application Of Multi-electrode Array In The Study Of Neuroinformatics & Neurophysiology

Multi-electrode Array has a plastic chamber, in which both brain slice andneural-network in vitro can be cultured on the surface of MEA. With the advantage ofthe combination of detailed insight into a single cell and enlarged view of research,MEA is qualified a very effective and practical method in diverse research areas.This dissertation begin with the active potential (AP) of neurons, which is theprincipium of signals detected by MEA system; methods of signal detection andsorting are involved. As a very reliable method, many research groups design specialtopographic forms of MEA to meat their special needs, and MEA are applied in manydifferent areas, here we briefly introduce some research hotspots and developingtrends of MEA. One target of researches carried on MEA is to study the NeuralCoding, especially the spatiotemporal coding of a single neuron and the currentlyheating theory of population coding as is introduced and studied in this dissertation.Then methods to improve the attachment of cultures on MEA are studied, we finallycultured olfactory and hippocampus neural system on the surface of MEA. The APsignal is detected by a self-designed high speed multi-channel system. Then wedesign a Matlab program to process the signals and pick up spikes. Based on theabove research, we studied the mode difference between neural systems with growthvariation through Raster analyses.The thesis utilizes a platform which is suitable for neural electrophysiology andneural biological studies by combining multi-electrode arrays and the self-designedneural signal analytical system.