An Experimental Study On Prevention Of Dura Mater Spinalis Adhssion After Laminectomy By Using KS Artificial Fasciae

Objective: To study the effects on prevention of dura mater spinalis adhesion afterlaminectomy through an experiment by using keratin substance (KS) artificial fasciae.Methods: Sixty rats were randomly divided into three groups, each had 20. In allrats, total laminectomy were performed at T₁₂ levels led to the defects of 2mm×8mm.The group A were sutured directly, which was regarded as control group; coveredwith artificial fasciae in group B; with self-faciae in group C. The animals were killed at2nd, 4th, 8th, 12th week after operation respectively, and macroscopy assessment was donewith the help of the Rydell Criterion. The spine segment of T₁₂ was removed en blocincluding the paraspinal tissue, and all specimens were stained with hematoxylin andeosin. Improved Nussbaum Criterion was used for the following optical microscopyassessment. Transmission electron microscopy was performed on posterior epidural scarobtained from group-B at the 2nd, 4th, 8th, 12th week after operation respectively. Thenonparametric Kruskal-Wallis test was used to determine the differences between thethree groups, and a P value of＜0.05 was considered statistically significant.Results: The use of the KS artificial fasciae caused no adverse effects. According tothe Rydell and improved Nussbaum Criterion A-group was statistically significantdifference from B and C-group (P＜0.05).However, no significant difference betweengroup B and C was observed(P＞0.05).So compared to the control team, KS artificialfasciae and self-fasciae significantly reduced the formation of epidural fibrosis afterlaminectomy.Conclusions: KS artificial fasciae have the best biological compatibility with thetissue with no adverse effects. As placement of KS artificial fasciae over thelaminectomy defect in rats could provide a physical barrier against invasion of fibroustissue into the vertebral canal, and peridural fibrosis after laminectomy defect in ratscould provide a physical barrier against invasion of fibrous tissue into the vertebral canal,and peridural fibrosis after laminectomy would be effectively prevented. KS artificialfasciae is safe and effective in the limiting the direct contact by the postlaminectomy epidural fibrosis in rats.