Concentration Variations Of Glutamate And γ-aminobutyric Acid In Frontal Cortex Of Uremia Encephalopathy Rat

Objective: To detect concentration variations of Glutamate andγ-aminobutyric acid infrontal cortex of acute renal failure rats with uremia encephalopathy and understand theralationship with uremia encephalopathy.Methods: 36 male healthy Wister rats were divided into 2 groups by random: 12 innormal control group, 24 in model group. In the model group, Cisplatin 7mg/kg i.p. wasadministered qd×2 to induce acute renal failure, the model group are observed for 7—14days. According to their neurologic manifestations, The model group was subdivided intouremia group and uremia encephalopathy group. Rats were decapitated and got outfrontal cortex quickly. High performance liquid chromatography (HPLC) was used todetect Glutamate andγ-aminobutyric acid contents in rat frontal cortex of the above 3groups. SCr was examined by automatic biochemistry analyzer.Results: The SCr level of uremia encephalopathy group was higher than thoseof control group and uremia group, has significant difference (p＜0.05). Concentrations ofglutamate andγ-aminobutyric acid in rat frontal cortex of uremia group and uremiaencephalopathy group changed. Compared with normal control group, concentrations ofGlutamate andγ-aminobutyric acid in uremia group show no significant difference(p＞0.05). Compared with normal control group and uremia group, concentrations ofGlutamate andγ-aminobutyric acid in uremia encephalopathy group shows significantdifference (p＜0.05). The concents of two neurotransmitters don't have linear correlationwith SCr (p＞0.05).Conclusion: The concentrations of Glutamate andγ-aminobutyric acid significantlyincrease when acute renal failure with uremia encephalopathy. SoGlutamate andγ-aminobutyric acid may play a great role in the mechanism of uremia encephalopathy.The SCr level of acute renal failure with uremia encephalopathy was higher than that ofacute renal failure without uremia encephalopathy. The concentrations of twoneurotransmitters don't have linear correlation with SCr, that is to say, higher level ofSCr doesn't mean higher concentrations of Glutamate andγ-aminobutyric acid in ratfrontal cortex.