The Significance Expression Of Matrix Metalloproteinase-9 In Human Renal Cell Carcinoma

Objective: To investigate the significance and expression of Matrix Metalloproteinase-9(MMP9) gene in human renal cell carcinoma. To understand the role of MMP9 in the course of renal cell carcinoma heterogeneity and the tumor occurrence and development.Methods: Reversal transcription polymerase chain reaction (RT-PCR) was used to assess the expression of MMP9 mRNA in 50 cases of renal cell carcinoma tissues and 10 cases of normal renal tissues far from neoplasm. Immunohistochemistry was used to assess the expression of MMP9 proteinum in 50 cases of renal cell carcinoma tissues and 10 cases of normal renal tissues far from neoplasm.Results: The average value of the expression of MMP9 mRNA was 0.42996±0.05996 in 50 RCC cases and 0.05048±0.02248 in control group. The expression of MMP9 in 50 RCC cases was significantly higher than that in control group (P=0.000＜0.01). And the expression of MMP9 increased if the RCC case was in a higher grade or stage. Immunohistochemistry showed that MMP9 was expressed in tumor cells and some stromal cells. Masculine particle was mainly expressed in cytoplasm and/or cellular nucleus. It also showed that positive expression of MMP9 proteinum was detected in 72% (36/50) of renal cell carcinoma tissues and in 20.00% (2/10)of nomal tissues and there was significant difference in epression of MMP9 proteinum between cancer tissues and nomal tissues (P=0.006＜0.05). There was significant difference in expression of MMP9 proteinum of different grades and stages of tumor (P＜0.05). The expression of MMP9 proteinum was up-regulated in the higher stage or grade.Conclusions: The expression of MMP9 in human RCC was higher than normal nephridial tissue and related to the staging and grading of RCC, which suggested that the expression of MMP9 was useful in the prognosis of RCC. This may provid theory basis of inhibition of MMP9 to carcinogenesis treatment of renal cell carcinoma.