Determination Of Uric Acid And Creatinine In Human Serum By Isotope Dilution Liquid Chromatography Mass Spectrometry

As an important indicator of many diseases, uric acid and creatinine have already aroused people's attention with the raise of living standard in recent years. Different techniques for the determination of uric acid and creatinine have been developed and applied in clinic laboratories.In this thesis, an ID/LC/MS method was developed to determine uric acid and creatinine in serum. [1, 3-¹⁵N₂]uric acid and D₃-creatinine were selected as labeled materials in the research. The conditions in serum sample pretreatment and the operation parameters of LC/MS were studied.At the optimized condition, the RSD of the uric acid and creatinine of three human serum samples by the ID/MS are less than 1%. Two NIST standard samples SRM 909b (â… ,â…¡) were used as certificated standards to evaluate the established method. The result showed that relative diviationes were less than+1%. The results obtained by this method were compared with that obtained by different columns and by LC/MS/MS with relative deviation less than±1%. The recoveries ranged from 99% to 101%. The total uncertainty of uric acid and creatinine of four human serum samples assays are less than 1%. The results showed that it could be hopefully recommended as reference method for uric acid and creatinine assays.