The Expression Of Myostatin In Smooth Muscle Of Human Bladder With UAD

To investigate whether and how myostatin(GDF-8)is expressed in smooth mucle of human bladder with Underactive detrusor(UAD).UAD is a common symptom a urologist could find himself facing. It can be the final result of central nervous system disease (Neurogenic bladder) or diabetes etc,It can also be the result of urine retention caused by bladder outlet obstruction(BOO) disease such as BPH.The common practice is to perform suprapubic cystostomy or urethral catheterization to prevent further harm to the kidney funtion,but that can cause huge uncomfort to the patient.How to solve this problem had always been the concern of uroloists throughout the world.To improve the contraction of detrusor might provide the final answer to this problem.But most research are directing toward how to improve the nerve control to bladder and the regulation of calcium gate way on smooth muscle cell in bladder.On the other hand ,Myostatin is a member of the transforming growth factor-βsuper family that encompasses a large group of growth and differentiation factors playing important roles in regulating embryonic development and in maintaining tissue homeostasis in adult animals.Almost all reseach on this factor is connected with skeletal muscle with a few exceptions on cardiac muscle.To observe the level of mRNA expression and protein,we used both RT-PCR and Western blot techniques which will help us to know if it is a key factor in the process of UAD forming.We hope our work will lay a solid foundation on the mechanism of UAD ,be benifitial for us to know how UAD develops and gather facts and information for further research on this factor. MethodsThe smooth muscle samples to test on were from 60 patients who performed open surgeries for BPH.A11 of the patients have taken the urodynamic examination. UAD was diagnosed by the linearized passive urethral resistance relation(LinPURR).Group A was defined as 20 patients with UAD and group B as 40 patients without UAD.The muscle samples were taken with the concents of patients .The level of mRNA of myostatin is measured through the RT-PCR method in which the cDNA amplifying product ofβ-actin was used as an internal reference. Western blot technique was used to detect the level of protein of myostatin in the muscle samples.All data were presented as x|-±s .t-test of the SPSS 11.0 for Windows was used for data analyzing. P<0.05 was considered to be statistically significant.ResultsBoth RT-PCR and Western blot are successful in detecting the existence of myostatin (protein/mRNA) in the samples,But the levels of mRNA and the product shows no statistical differences between the two groups with an mRNA level 135.5%±34.7% to 120.6%±19.0%.and product level 48.5±8.2 to 44.2±7.8 respectively.Conclusions1 .Myostatin existed in the smooth muscle of human bladder although its function is still not clear. 2.The levels of myostatin mRNA and the product show no statistical difference between UAD and non-UAD patients in their bladder smooth muscle.3.Myostatin might not be an important regulator in smooth muscle in the development of UAD.