The Effects Of Propofol On The Expression Of NF-κB,TNF-a And IL-6 During Acute Myocardial Ischemia-reperfusion Injury In Rats

Objective To study the effects of different doses of propofol on the production of nuclear factor-κB(NF-κB),tumor necrosis factor- a(TNF-a)and interleukin-6(IL-6)following myocardial ischemia-reperfusion injury of rats,and to investigate its mechanism.Methods One hundred twenty rats were divided into four groups:①Sham group②Ischemia-Reperfusion(I/R)group③Low dose group④High dose group.The myocardial samples were studied at 30 min after ischemia and 30,60,120,240 min reperfusion after ischemia.The exprement deployed immunohistochemistry(IHC)in order to detect qualitalively and semiquantitatively the express of NF-κB,and radio-immunity(RI)in order to detect quantitative the level of TNF-a and IL-6.Results In IR group,LD group and HD group,after ischemia for 30 min,the level of NF-κB was increased and reached a peak at 60 min after reperfusion,and kept the high level. The level TNF-a and IL-6 were increased after ischemia for 30 min and reached a peak at 60 min after reperfusion,and maintained the high level.In LD group and HD group,propofol could inhibit the activation of NF-κB and expression of TNF-a and IL-6,by which it protected myocardial function.But low dose group was better than high dose group.Conclusion Propofol could inhibit I/R- indused increase in NF-κB,TNF-a and IL-6 expression.And low dose group was better than high dose group.