Growth Inhibition Effect Of Cinobufacini On Cholangiocarcinoma Cell

Background/Purpose: In this experiment we used cinobufacini to investigated it's growth inhibition effect on cholangiocarcinoma cell QBC939 in vitro and possible mechanisms..Methods: Cholangiocarcinoma cell line QBC939 were cultured in vitro. MTT assay were used to determineted the antiproliferative effect of different doses cinobufacini on cholangiocarcinoma cell QBC939 and growth rate were calculated. Apoptosis in the cholangiocarcinoma cell QBC939 after dfferent doses cinobufacini administration were evaluated by flow cytometry. Effect of cinobufacini on the expression of caspase-3 and Fas mRNA were evaluated by RT-PCR.Results: MTT assay showed that significantly reduced QBC939 cell growth and were dose-dependent.After treated by 0.01～10ug/ml cinobufacini for 48 hours, the cell inhibitory rate increased gradually,respectively 26.8 % , 30% , 56.5%, 71% .Between control group and experimental group the OD value was significant different (p< 0.001). Result of the flow cytometry showed that cinobufacini can induce cell apoptosis . Expression of Fas, caspase-3 mRNA was depended on doses of cinobufacini . Cinobufacini can increase its expression.Conclusion: The result proved that cinobufacini inhibits the proliferation and induct apoptosis of human extrahepatic cholangiocarcinoma cell line QBC939 .The mechanism of it's effect was cell apoptosis . The up-regulation of Fas, caspase-3 mRNA might be correlated with cell apoptosis induction.